This study evaluates the effect of multimodal ultrasound on the extraction efficiency and immunoregulatory activity of polysaccharides from Lepidium meyenii Walp. (LMP). The separation and purification of maca polysaccharides were investigated by the DEAE-52 cellulose column, and the monosaccharide compositions were identified by HPGPC. Their immune activity was analyzed by the secretion of cytokines (TNF-α and IL-6) from RAW 264.7 macrophage. The results showed that the optimal extraction conditions were energy aggregation alternation dual-frequency ultrasound (EADU) with frequency combinations of 20/35, extraction time of 15 min, material/water ratio of 1:10 g/mL, ultrasonic power intensity of 150 W/L, intermittent time ratio of 4 s/3 s, and extraction temperature of 50 ℃. The extraction rates of purified polysaccharides (US3) increased by 44.90%. The LMP extracted by EADU contained arabinose, galactose, and glucose in the molar ratios of 2.9:2.72:5.05. In addition, US3 promoted the release of TNF-α and IL-6 from RAW 264.7 better than RS3 (purified polysaccharides extracted by hot water), which indicated that US3 exerted remarkable immune activity. It could be an excellent functional additive in food or medicine.

本研究评估了多模态超声对马齿苋多糖提取效率和免疫调节活性的影响沃尔普。(LMP)。DEAE-52纤维素柱研究了玛咖多糖的分离纯化，HPGPC鉴定了单糖组成。通过从 RAW 264.7 巨噬细胞分泌细胞因子（TNF-α 和 IL-6）来分析它们的免疫活性。结果表明,最佳提取条件为能量聚集交替双频超声(EADU),频率组合为20/35,提取时间为15 min,料水比为1:10 g/mL,超声功率为150 W/L，间歇时间比4 s/3 s，萃取温度50 ℃。纯化多糖（US3）的提取率提高了44.90%。EADU提取的LMP含有摩尔比为2.9:2.72:5.05的阿拉伯糖、半乳糖和葡萄糖。此外，US3对RAW 264.7释放TNF-α和IL-6的促进作用优于RS3（热水提取纯化多糖），表明US3具有显着的免疫活性。它可能是食品或药物中极好的功能性添加剂。