At present, the development of high-performance protein imprinted materials is still a research hotspot in the field of protein imprinting. Herein, anti-protein adsorption segment (CBMA)-assisted self-driven bovine serum albumin (BSA) surface imprinted fibers MTCFs@SIP@CBMA with high recognition selectivity are pioneered using the strategies of combining magnetic nanomaterial surface imprinting technique with amino-Michael addition. The special structure of the carrier MTCFs endows MTCFs@SIP@CBMA with magnetic performance and self-driven adsorption performance, which simplifies the separation process while improving the adsorption capacity and accelerating the adsorption rate. The adsorption capacity for BSA reached 390 ± 20 mg/g within 30 min. The introduction of CBMA segments on the surface after imprinting by amino-Michael addition makes its polymer chain length and position controllable. Under the strongest anti-nonspecific adsorption effect, MTCFs@SIP@CBMA exhibit excellent specific identification to BSA from mixed proteins. Additionally, MTCFs@SIP@CBMA show considerable reusability. Therefore, MTCFs@SIP@CBMA are expected to be applied in efficient separation of proteins in biological samples.

中文翻译：

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抗非特异性吸附片段辅助自驱动表面印迹纤维用于高效蛋白质分离

目前，高性能蛋白质印迹材料的开发仍是蛋白质印迹领域的研究热点。本文采用磁性纳米材料表面印迹技术与氨基-迈克尔加成相结合的策略，开创了具有高识别选择性的抗蛋白吸附片段（CBMA）辅助自驱动牛血清白蛋白（BSA）表面印迹纤维MTCFs@SIP@CBMA . 载体MTCFs的特殊结构赋予了MTCFs@SIP@CBMA磁性能和自驱动吸附性能，在简化分离过程的同时提高了吸附容量，加快了吸附速率。BSA 的吸附量在 30 分钟内达到 390 ± 20 mg/g。通过氨基-迈克尔加成压印后在表面引入CBMA链段，使其聚合物链长和位置可控。在最强的抗非特异性吸附作用下，MTCFs@SIP@CBMA对混合蛋白中的BSA表现出优异的特异性识别。此外，MTCFs@SIP@CBMA 显示出相当大的可重用性。因此，MTCFs@SIP@CBMA有望应用于生物样品中蛋白质的高效分离。