Funding Acknowledgements Type of funding sources: Public Institution(s). Main funding source(s): O.O.Bogomolets National Medical University Relevance: Amino acids are one of the important group of myocardial metabolites. Today metabolomic analysis has become an essential tool for understanding the pathophysiology of cardiovascular disorders [1, 3]. Coagulation factors interact with platelets by binding to platelet receptors directly or indirectly or by cleaving of the platelet receptors. Arterial thrombosis as a most life threating atherosclerosis (AS) complication is characterized by significant interplay between platelets and coagulation [2]. Therefore, it is important to find pathogenic links between changes in platelet structure and coagulation data. Materials and methods 78 patients were investigated. All patients were divided into two groups – 20 patients without AS (control group) and 58 patients with AS (mean group). Diagnosis was verified by carotid and peripheral arteries ultrasound. Statistically all groups were compatible by the main clinical features (age, gender, concominant disorders, etc.). For amino acid platelet determination, platelet-rich plasma was obtained after blood centrifugation at 3000 g 15 min of 10 ml of the remaining plasma. One milliliter of platelet-rich plasma was centrifuged at 2,500 g for 15 min to obtain a platelet pellet that was frozen at –20°C until assayed. All the samples were stored for a maximum of 3 mounth. Platelets amino acid spectrum was checked by ion exchange liquid column gas chromatography. Results Patients of the mean group has significantly differences compared with control group: decreasing of taurine (6.54±9.49 vs 5.94±4.36 mkmol/107), threonine (0.48±1.69 vs 0.22±1.24 mkmol/107), glycine (2.10±3.79 vs 1.02±2.11 mkmol/107) and glutamine (2.54±5.70 vs 1.88±2.76 mkmol/107) levels; increasing alanine (1.99±4.03 vs 2.81±3.06 mkmol/107), valine (0.96±1.98 vs 1.63±1.26 mkmol/107) and isoleucine (0.53±1.84 vs 2.91±1.61 mkmol/107) levels. Also in mean group acquired leucine (2.51±0.93 mkmol/107) that was not present in control group. Total amount of branch-chained amino acids (BCAA) in platelets is also increased significantly. Therefore, in mean group patients were significant higher-level activated partial thromboplastin time (APTT) (34.3±2.87 vs 28.8±3.29 s) and lower fibrinogen (205±17 vs 213±2 mg/dl). Direct significant correlation was checked between glycine (r=0,634; p<0,05), alanine (r=0,587; p<0,05) and fibrinogen, also between APTT and platelets BCAA (r=0,724; p<0,01). Conclusion We obtained reliable changes in platelets amino acid spectrum and coagulation tests in patients with atherosclerosis. In addition, significant correlations between themes can be related with pathogenic platelets changes. In any case more deeper studies are desirable.

中文翻译：

---

动脉粥样硬化患者的血小板氨基酸谱和凝血

资金致谢 资金来源类型：公共机构。主要资金来源：OOBogomolets 国立医科大学相关性：氨基酸是心肌代谢物的重要组之一。如今，代谢组学分析已成为了解心血管疾病病理生理学的重要工具 [1, 3]。凝血因子通过直接或间接结合血小板受体或通过切割血小板受体与血小板相互作用。动脉血栓形成是最威胁生命的动脉粥样硬化 (AS) 并发症，其特点是血小板和凝血之间存在显着的相互作用 [2]。因此，重要的是要找到血小板结构变化和凝血数据之间的致病联系。材料和方法 78 名患者进行了调查。所有患者分为两组——20 例无 AS 患者（对照组）和 58 例 AS 患者（平均组）。诊断通过颈动脉和外周动脉超声验证。从统计学上讲，所有组的主要临床特征（年龄、性别、伴随疾病等）都是一致的。对于氨基酸血小板测定，在将 10 ml 剩余血浆以 3000 g 离心 15 分钟后获得富含血小板的血浆。将 1 毫升富含血小板的血浆以 2,500 g 的速度离心 15 分钟以获得血小板颗粒，将其冷冻在 –20°C 直至进行检测。所有样品最多保存 3 个月。通过离子交换液相柱气相色谱检查血小板氨基酸谱。结果平均组患者与对照组相比有显着差异：牛磺酸（6.54±9.49 vs 5.94±4.36 mkmol/107）、苏氨酸（0.48±1.69 vs 0.22±1.24 mkmol/107）、甘氨酸（2.10±3.79 vs 1.02±2.11 mkmol/107）和谷氨酰胺（2.54±5.70 vs. 1.88±2.76 mkmol/107) 水平；增加丙氨酸（1.99±4.03 vs 2.81±3.06 mkmol/107）、缬氨酸（0.96±1.98 vs 1.63±1.26 mkmol/107）和异亮氨酸（0.53±1.84 vs 2.91±1.61 mkmol/107）水平。同样在平均组中获得了对照组中不存在的亮氨酸（2.51±0.93 mkmol/107）。血小板中支链氨基酸（BCAA）的总量也显着增加。因此，在平均组中，患者的活化部分促凝血酶原激酶时间（APTT）显着较高（34.3±2.87 vs 28.8±3.29 s）和较低的纤维蛋白原（205±17 vs 213±2 mg/dl）。在甘氨酸 (r=0,634; p<0,05)、丙氨酸 (r=0,587; p<0, 05) 和纤维蛋白原，也在 APTT 和血小板 BCAA 之间 (r=0,724；p<0,01)。结论 我们获得了动脉粥样硬化患者血小板氨基酸谱和凝血试验的可靠变化。此外，主题之间的显着相关性可能与致病性血小板变化有关。无论如何，更深入的研究是可取的。