Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Karl Landsteiner Institute Cardiovascular Research Background Saline is still the most widely used storage and flushing solution in cardiovascular procedures despite knowing evidence of its influence on the human endothelial cell function. Aim of this study was to assess the effect of DuraGraft©, an intraoperative graft treatment solution, on human saphenous vein segments, rat aortic segments and human umbilical vein endothelial cells (HUVECs) in comparison to saline. Methods Within 12 patients undergoing aortocoronary bypass surgery, saphenous vein graft segments were randomized to DuraGraft© (n=12/6) or saline (n=12/6) solution before intraoperative storage. These segments as well as rat aortic segments underwent assessment of vascular function in a multichamber isometric myograph system in comparison to Krebs-Henseleit solution (KHS), a physiologic organ buffer solution. Additionally, human umbilical vein endothelial cells (HUVECs) were used for cell viability tests. Results KCl-induced contraction showed a tendency toward increase when treated with DuraGraft© compared to normal saline preservation of human vein segments (24.73±16.22 vs. 15.59±9.53 N/m2, P Conclusion DuraGraft© demonstrated a favorable effect on graft relaxation and contraction indicating preservation of vascular endothelial function. Saline is clearly not only inferior to this specialized solution but may show additional harmful effects to viability.

中文翻译：

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血管移植物储存溶液保留内皮功能

资金致谢 资金来源类型：公共拨款——仅限国家预算。主要资金来源：Karl Landsteiner Institute 心血管研究 背景 生理盐水仍然是心血管手术中使用最广泛的储存和冲洗溶液，尽管有证据表明它对人体内皮细胞功能有影响。本研究的目的是评估 DuraGraft©（一种术中移植物治疗溶液）与盐水相比对人大隐静脉段、大鼠主动脉段和人脐静脉内皮细胞 (HUVEC) 的影响。方法 12例接受主动脉冠状动脉搭桥手术的患者，在术中储存前，将大隐静脉移植节段随机分配至DuraGraft©（n=12/6）或生理盐水（n=12/6）溶液。与生理器官缓冲溶液 Krebs-Henseleit 溶液 (KHS) 相比，这些节段以及大鼠主动脉节段在多腔等距肌动描记系统中对血管功能进行了评估。此外，人脐静脉内皮细胞 (HUVEC) 用于细胞活力测试。结果 与生理盐水保存的人静脉段相比，用 DuraGraft© 治疗 KCl 诱导的收缩有增加的趋势（24.73±16.22 对 15.59±9.53 N/m2，P 结论 DuraGraft© 对移植物松弛和收缩有良好的效果表明保留了血管内皮功能。盐水显然不仅不如这种专门的溶液，而且可能对生存能力产生额外的有害影响。