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Feulgen Testing of the Nuclei of Leaf Cells of Taxodium dubium (Cupressaceae) from the Eocene Tavda Flora of Western Siberia
Paleontological Journal ( IF 0.7 ) Pub Date : 2022-06-13 , DOI: 10.1134/s0031030122030108
I. A. Ozerov , N. A. Zhinkina , A. A. Torshilova , E. M. Machs , A. V. Rodionov

Abstract

The article presents the results of studying the tissues of the leaf phytoleim of the fossil Taxodium dubium from the Eocene Tavda flora of Western Siberia. Feulgen testing showed the preservation of Feulgen-positive, heterogeneously stained nuclear structures and small granular chromocenters in the cells. The nuclei were red–violet-stained and chromocenters were stained more intensely. This indicates the occurrence of aldehyde groups of deoxyribose in the nuclear chromatin and demonstrates the specific presence of DNA. Additional staining of the preparations with alcian blue and Ehrlich’s hematoxylin displays cell walls and the adjacent intracellular material.



中文翻译:

西西伯利亚始新世 Tavda Flora 紫杉 (柏科) 叶细胞核的 Feulgen 测试

摘要

本文介绍了研究西西伯利亚始新世塔夫达植物群化石Taxodium dubium的叶植物组织的结果。Feulgen 测试显示在细胞中保留了 Feulgen 阳性、不均匀染色的核结构和小颗粒染色中心。细胞核呈红紫色染色,染色中心染色更强烈。这表明核染色质中存在脱氧核糖的醛基,并证明了 DNA 的特异性存在。用爱新蓝和埃利希苏木精对制剂进行额外染色显示细胞壁和相邻的细胞内物质。

更新日期:2022-06-14
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