In this study, reduced graphene oxide with immobilized silver nanoparticles cotton fabric (Ag/rGO/cotton) was produced by the dip-coating cotton in silver immobilizing onto graphene oxide (Ag/GO) suspension to prepared Ag/GO/cotton material followed by the addition of vitamin C (VC) as an environmentally friendly reducing agent. The characteristics of Ag/GO and modified cotton were investigated by Fourier transform infrared spectroscopy, X-ray diffraction, Raman spectroscopy, transmission electron microscopy, scanning electron microscope, X-ray photoelectron spectroscopy, and energy-dispersive X-ray spectroscopy. Silver nanoparticles (AgNPs) were uniformly distributed on the surface of graphene oxide (GO) sheets with an average size of 10–15 nm, while the cotton surface was evenly covered by Ag/rGO. The zone of inhibition against Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), and Escherichia coli (E. coli) bacteria indicated that Ag/rGO/cotton possessed the highest antibacterial activity when compared to other modified cotton. Moreover, the Ag/rGO/cotton also exhibited effective hydrophobicity with a wetting angle of 103.85° ± 0.75°, which supported the prevention of bacterial infection and adherent on the cotton surface. To confirm the low cytotoxic property of Ag/rGO/cotton for human use, the cell viability of HepG2, A549, and Hek293 cell lines were evaluated when contacted with the material, while the low amount of leached Ag⁺ from Ag/rGO/cotton was under the accepted limit. All results of the study confirmed that Ag/rGO/cotton possesses significant potential for several antibacterial applications such as protective equipment.

Graphic abstract

中文翻译：

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涂有石墨烯基银纳米粒子的棉织物：合成、改性和抗菌活性

在这项研究中，通过将银中的浸涂棉固定在氧化石墨烯 (Ag/GO) 悬浮液上制备 Ag/GO/棉材料，制备了具有固定银纳米粒子棉织物 (Ag/rGO/cotton) 的还原氧化石墨烯。添加维生素C（VC）作为环保还原剂​​。通过傅里叶变换红外光谱、X射线衍射、拉曼光谱、透射电子显微镜、扫描电子显微镜、X射线光电子能谱和能量色散X射线光谱研究了Ag/GO和改性棉的特性。银纳米粒子 (AgNPs) 均匀分布在氧化石墨烯 (GO) 片材的表面，平均尺寸为 10-15 nm，而棉花表面均匀地被 Ag/rGO 覆盖。抑制区金黄色葡萄球菌（S. aureus）、铜绿假单胞菌（P. aeruginosa）和大肠杆菌（E. coli）细菌表明Ag/rGO/棉与其他改性棉相比具有最高的抗菌活性。此外，Ag/rGO/棉还表现出有效的疏水性，润湿角为 103.85°±0.75°，有助于防止细菌感染并粘附在棉花表面。为了确认 Ag/rGO/棉对人类使用的低细胞毒性，HepG2、A549 和 Hek293 细胞系在与材料接触时的细胞活力进行了评估，而浸出的少量 Ag ⁺来自 Ag/rGO/cotton 的产品低于可接受的限度。研究的所有结果都证实，Ag/rGO/棉在防护设备等多种抗菌应用方面具有巨大潜力。

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