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Efficient conversion of hemicellulose into 2, 3-butanediol by engineered psychrotrophic Raoultella terrigena: mechanism and efficiency
Bioresource Technology ( IF 9.7 ) Pub Date : 2022-06-11 , DOI: 10.1016/j.biortech.2022.127453 Yue Wang 1 , Bowen Wu 1 , Tian Ma 1 , Yaozu Mi 1 , Hanyi Jiang 1 , Haohao Yan 1 , Peichao Zhao 1 , Shuo Zhang 1 , Linxuan Wu 1 , Lei Chen 1 , Hailian Zang 1 , Chunyan Li 1
Bioresource Technology ( IF 9.7 ) Pub Date : 2022-06-11 , DOI: 10.1016/j.biortech.2022.127453 Yue Wang 1 , Bowen Wu 1 , Tian Ma 1 , Yaozu Mi 1 , Hanyi Jiang 1 , Haohao Yan 1 , Peichao Zhao 1 , Shuo Zhang 1 , Linxuan Wu 1 , Lei Chen 1 , Hailian Zang 1 , Chunyan Li 1
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Low-temperature biorefineries inhibit the multiplication of undesired microorganisms, improve product purity and reduce economic costs. Herein, to improve the 2,3-butanediol (2,3-BD) bioconversion efficiency from hemicellulose, a psychrotrophic hemicellulose-degrading strain HC6 with high β-xylosidase activity 1520 U/mL was isolated and genetically modified. Xylan (hemicellulose replacement) was depolymerized into xylooligosaccharides (XOS) and xylose by HC6, which were further converted into 2,3-BD. Transcriptomic analysis revealed that β-xylosidase gene () and xylose isomerase gene (), which are beneficial for increasing the carbon flux from xylan to 2,3-BD, were significantly upregulated 56.9-fold and 234-fold, respectively. A recombinant strain was constructed by overexpressing in HC6, which obtained 0.389 g/g yield of 2,3-BD from hemicellulose extracted from corn straw at 15 °C. This study proposed a promised strategy for the bioconversion of agricultural waste into 2,3-BD at low temperatures and provides a basis for future efforts in the achievement of carbon neutrality.
中文翻译：
通过工程耐冷 Raoultella terrigena 将半纤维素有效转化为 2, 3-丁二醇：机制和效率
低温生物精炼厂可抑制不良微生物的繁殖，提高产品纯度并降低经济成本。在此，为了提高半纤维素生物转化2,3-丁二醇（2,3-BD）的效率，分离并遗传改造了具有高β-木糖苷酶活性1520 U/mL的耐冷半纤维素降解菌株HC6。木聚糖（半纤维素替代物）被HC6解聚为低聚木糖（XOS）和木糖，并进一步转化为2,3-BD。转录组分析显示，有利于增加从木聚糖到2,3-BD的碳通量的β-木糖苷酶基因()和木糖异构酶基因()分别显着上调56.9倍和234倍。通过在HC6中过表达构建了重组菌株，在15℃下从玉米秸秆中提取的半纤维素获得了0.389 g/g的2,3-BD产量。这项研究提出了一种在低温下将农业废物生物转化为2,3-BD的承诺策略，并为未来实现碳中和的努力奠定了基础。
更新日期：2022-06-11
中文翻译：
通过工程耐冷 Raoultella terrigena 将半纤维素有效转化为 2, 3-丁二醇：机制和效率
低温生物精炼厂可抑制不良微生物的繁殖，提高产品纯度并降低经济成本。在此，为了提高半纤维素生物转化2,3-丁二醇（2,3-BD）的效率，分离并遗传改造了具有高β-木糖苷酶活性1520 U/mL的耐冷半纤维素降解菌株HC6。木聚糖（半纤维素替代物）被HC6解聚为低聚木糖（XOS）和木糖，并进一步转化为2,3-BD。转录组分析显示，有利于增加从木聚糖到2,3-BD的碳通量的β-木糖苷酶基因()和木糖异构酶基因()分别显着上调56.9倍和234倍。通过在HC6中过表达构建了重组菌株，在15℃下从玉米秸秆中提取的半纤维素获得了0.389 g/g的2,3-BD产量。这项研究提出了一种在低温下将农业废物生物转化为2,3-BD的承诺策略，并为未来实现碳中和的努力奠定了基础。
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