In this work, novel luminol functionalized nanoparticles (SnO₂@Luminol NPs) with mesoporous spherical structure were synthesized by a simple and green method, which exhibited good chemiluminescence (CL) intensity without any additional catalysts. In addition, the CL intensities generated by SnO₂@Luminol/H₂O₂ system were linearly related to the logarithm values of H₂O₂ concentrations, and CL mechanism of SnO₂@Luminol/H₂O₂ system was investigated. On this basis, a sensitive CL method was proposed to determine glucose and uric acid. The fabricated CL method displayed wide linear ranges (glucose, 1.0 × 10⁻⁸ – 1.0 × 10⁻⁴ M; uric acid, 1.0 × 10⁻⁸ – 1.0 × 10⁻⁴ M) and low detection limits (glucose, 5.4 nM; uric acid, 6.8 nM). The proposed CL method achieved sensitive and specific glucose and uric acid detection in human serum specimens, and the measured results were consistent with the clinical diagnosis. Furthermore, as long as the product of reaction between enzyme and substrate is H₂O₂, this CL method can also be extended to other biological detection fields.

在这项工作中，通过简单且绿色的方法合成了具有介孔球形结构的新型鲁米诺功能化纳米粒子（SnO _{2 @Luminol NPs），该纳米粒子在没有任何额外催化剂的情况下表现出良好的化学发光（CL）强度。}此外，SnO ₂ @Luminol/H ₂ O _{2体系产生的发光强度与H 2} O ₂浓度的对数值呈线性关系，研究了SnO ₂ @Luminol/H ₂ O ₂体系的发光机理。在此基础上，提出了一种灵敏的CL法测定葡萄糖和尿酸。制造的 CL 方法显示出较宽的线性范围（葡萄糖，1.0 × 10^-8 – 1.0 × 10 ^-4  M；尿酸，1.0 × 10 ^-8 – 1.0 × 10 ^-4  M）和低检测限（葡萄糖，5.4 nM；尿酸，6.8 nM）。所提出的CL方法实现了对人血清标本中葡萄糖和尿酸的灵敏特异的检测，测量结果与临床诊断一致。此外，只要酶与底物反应的产物是H ₂ O ₂，​​这种CL方法也可以扩展到其他生物检测领域。