Recently, increasing attention has been paid to antibiotic resistance genes (ARGs) in urban stormwater runoff. However, no available literature could be found on ARGs leaching from urban underlying surface sediments during stormwater runoff. In this study, surface sediments from commercial public squares around Nanjing (China) was selected for the investigation of target ARGs leaching kinetics, showing that absolute abundances of target ARGs desorption reached at the equilibrium during leaching time of 120-240min with all of the desorption efficiencies about 30%, indicating that there would be low proportion of leaching ARGs in the total ARGs migrating with runoff during rainfall events. Five target ARGs leaching including intI1 (clinic), strA, strB, tetM and tetX can be better described by the pseudo-second-order equation, while qacEdelta1 leaching can be better described by the pseudo-first-order equation, and the leaching for both sul1 and sul2 can be well described by the pseudo-first-order and pseudo-second-order equations. The effects of environmental factors including S/L ratios, pH values and water temperatures indicated that leaching efficiencies of target ARGs enhanced significantly with the increase of S/L ratios and water temperatures, but decreased with the increase of pH values. The transmission experiments after the simulated solar irradiation and heat implied that both large solar UV irradiation (30W/m²) and high temperature (40℃) were conducive to conjugation and transformation frequencies of ARGs. Furthermore, both high Cao and medium PAM levels could be effective for blocking ARGs transmission in the leachate from underlying surface sediments.

最近，城市雨水径流中的抗生素抗性基因（ARGs）受到越来越多的关注。然而，没有关于雨水径流期间从城市下层地表沉积物中浸出的 ARGs 的可用文献。本研究选取南京周边商业公共广场的表层沉积物进行目标 ARGs 浸出动力学研究，结果表明目标 ARGs 解吸的绝对丰度在 120-240 分钟的浸出时间达到平衡，所有解吸效率约为 30%，表明在降雨事件期间随径流迁移的总 ARGs 中淋溶 ARGs 的比例较低。五个目标 ARG 浸出，包括int I1 (clinic)、str A、str B、tet M 和tet X 可以更好地用伪二阶方程来描述，而qac Edelta1 浸出可以更好地用伪一阶方程来描述，并且sul 1 和sul 2 的浸出可以很好地描述为伪一阶和伪二阶方程。S/L比、pH值和水温等环境因素的影响表明，目标ARGs的浸出效率随着S/L比和水温的升高而显着增强，但随着pH值的升高而降低。模拟太阳辐照和热量后的透射实验表明，无论是大太阳紫外辐照（30W/m ²）和高温（40℃）有利于ARGs的共轭和转化频率。此外，高 Cao 和中等 PAM 水平均可有效阻止 ARGs 从下伏地表沉积物渗滤液中传播。