Microplastics, or nanoplastics fragmented to sizes in the nanoscale, can easily penetrate living organisms as well as human organs, increasing the risk of toxicity. However, it is challenging to obtain the size of nanoplastics using thermal analysis methods such as pyrolysis gas chromatography/mass spectrometry or thermal desorption-gas chromatography/mass spectrometry, which are used to analyze nanoplastics. In this study, the coupling effect due to the aggregation of gold nanoparticles (AuNPs) was used to measure the concentration of polystyrene nanoplastics (PSNPs). Experiments were conducted to measure the concentration of PSNPs using an ultraviolet—visible spectrophotometer using the phenomenon that the color of the colloid changes when AuNPs are aggregated. The differences in absorbance before and after aggregation after the addition of NaCl were measured. As a result of the experiment, when 20 mM NaCl was added to the solution in which AuNPs and PSNPs were dispersed, the difference in absorbance before and after aggregation and the concentration of PSNPs exhibited high linearity. In addition, 350 and 880 nm-sized PSNPs could be distinguished from each other because of their different linearities. The concentration of PSNPs was measured easily and conveniently without requiring a skilled operator, expensive analytical equipment; additionally, the process was not time or labor intensive, and it was shown that particle size can be measured by distinguishing particles of different sizes.

微塑料或碎裂成纳米级大小的纳米塑料很容易穿透生物体和人体器官，从而增加毒性风险。然而，使用热解气相色谱/质谱法或热解吸-气相色谱/质谱法等用于分析纳米塑料的热分析方法获得纳米塑料的尺寸具有挑战性。在这项研究中，由于金纳米粒子（AuNPs）的聚集产生的耦合效应被用来测量聚苯乙烯纳米塑料（PSNPs）的浓度。利用紫外-可见分光光度计，利用AuNPs聚集时胶体颜色发生变化的现象，进行了实验以测量PSNPs的浓度。测量加入NaCl后聚集前后的吸光度差异。实验结果表明，当向分散有AuNPs和PSNPs的溶液中加入20mM NaCl时，聚集前后的吸光度差异和PSNPs的浓度呈现出较高的线性关系。此外，350 和 880 nm 大小的 PSNP 由于线性不同而可以相互区分。无需熟练的操作员和昂贵的分析设备即可轻松方便地测量 PSNPs 的浓度；此外，该过程不是时间或劳动密集型的，并且表明可以通过区分不同尺寸的颗粒来测量粒度。当向分散有AuNPs和PSNPs的溶液中加入20 mM NaCl时，聚集前后的吸光度差异和PSNPs的浓度表现出较高的线性关系。此外，350 和 880 nm 大小的 PSNP 由于线性不同而可以相互区分。无需熟练的操作员和昂贵的分析设备即可轻松方便地测量 PSNPs 的浓度；此外，该过程不是时间或劳动密集型的，并且表明可以通过区分不同尺寸的颗粒来测量粒度。当向分散有AuNPs和PSNPs的溶液中加入20 mM NaCl时，聚集前后的吸光度差异和PSNPs的浓度表现出较高的线性关系。此外，350 和 880 nm 大小的 PSNP 由于线性不同而可以相互区分。无需熟练的操作员和昂贵的分析设备即可轻松方便地测量 PSNPs 的浓度；此外，该过程不是时间或劳动密集型的，并且表明可以通过区分不同尺寸的颗粒来测量粒度。无需熟练的操作员和昂贵的分析设备即可轻松方便地测量 PSNPs 的浓度；此外，该过程不是时间或劳动密集型的，并且表明可以通过区分不同尺寸的颗粒来测量粒度。无需熟练的操作员和昂贵的分析设备即可轻松方便地测量 PSNPs 的浓度；此外，该过程不是时间或劳动密集型的，并且表明可以通过区分不同尺寸的颗粒来测量粒度。