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Co-silencing of ABA receptors (SlRCAR) reveals interactions between ABA and ethylene signaling during tomato fruit ripening
Horticulture Research ( IF 7.6 ) Pub Date : 2022-06-07 , DOI: 10.1093/hr/uhac057
Jian Zou 1 , Ning Li 1 , Nan Hu 1 , Ning Tang 1 , Haohao Cao 1 , Yudong Liu 1 , Jing Chen 1 , Wei Jian 1 , Yanqiang Gao 1 , Jun Yang 2 , Zhengguo Li 1
Affiliation  

The ripening of fleshy fruits is highly dependent on the regulation of endogenous hormones, including ethylene, abscisic acid (ABA) and other phytohormones. However, the regulatory mechanism of ABA signaling and its interaction with ethylene signaling in fruit ripening are still unclear. In this study, multi-gene interference (RNAi) was applied to silence the ABA receptor genes in tomato for screening the specific receptors that mediate ABA signaling during fruit ripening. The results indicated that the ABA receptors, including SlRCAR9, SlRCAR12, SlRCAR11, and SlRCAR13, participate in the regulation of tomato fruit ripening. Comparative analysis showed that SlRCAR11 and SlRCAR13 play more important roles in mediating ABA signaling during tomato fruit ripening. Co-silencing of the four genes encoding these receptors could weaken the ethylene biosynthesis and signaling pathway at the early stage of tomato fruit ripening, leading to delayed fruit ripening. Meanwhile, co-silencing enhanced fruit firmness, and altered the shelf-life and susceptibility to Botrytis cinerea of the transgenic fruits. Furthermore, blocking ABA signaling did not affect the ability of ethylene to induce fruit ripening, whereas the block may inhibit the effectiveness of ABA in promoting fruit ripening. These results suggested that ABA signaling may be located upstream of ethylene signaling in regulating fruit ripening. Our findings provide a new insight into the complex regulatory network of phytohormones in regulating fruit ripening in tomato.

中文翻译:

ABA 受体 (SlRCAR) 的共沉默揭示了番茄果实成熟过程中 ABA 和乙烯信号之间的相互作用

肉质果实的成熟高度依赖于内源激素的调节,包括乙烯、脱落酸(ABA)和其他植物激素。然而,ABA信号的调控机制及其与乙烯信号在果实成熟过程中的相互作用仍不清楚。在这项研究中,应用多基因干扰 (RNAi) 沉默番茄中的 ABA 受体基因,以筛选在果实成熟过程中介导 ABA 信号传导的特定受体。结果表明,ABA受体包括SlRCAR9、SlRCAR12、SlRCAR11和SlRCAR13参与了番茄果实成熟的调控。对比分析表明,SlRCAR11和SlRCAR13在番茄果实成熟过程中介导ABA信号传导的作用更为重要。编码这些受体的四个基因的共沉默可能会削弱番茄果实成熟早期的乙烯生物合成和信号通路,导致果实成熟延迟。同时,共沉默增强了果实的硬度,并改变了转基因果实的保质期和对灰霉病的易感性。此外,阻断ABA信号不影响乙烯诱导果实成熟的能力,而阻断可能抑制ABA促进果实成熟的有效性。这些结果表明ABA信号可能位于乙烯信号的上游调节果实成熟。我们的研究结果为植物激素在调节番茄果实成熟中的复杂调控网络提供了新的见解。导致果实成熟延迟。同时,共沉默增强了果实的硬度,并改变了转基因果实的保质期和对灰霉病的易感性。此外,阻断ABA信号不影响乙烯诱导果实成熟的能力,而阻断可能抑制ABA促进果实成熟的有效性。这些结果表明ABA信号可能位于乙烯信号的上游调节果实成熟。我们的研究结果为植物激素在调节番茄果实成熟中的复杂调控网络提供了新的见解。导致果实成熟延迟。同时,共沉默增强了果实的硬度,并改变了转基因果实的保质期和对灰霉病的易感性。此外,阻断ABA信号不影响乙烯诱导果实成熟的能力,而阻断可能抑制ABA促进果实成熟的有效性。这些结果表明ABA信号可能位于乙烯信号的上游调节果实成熟。我们的研究结果为植物激素在调节番茄果实成熟中的复杂调控网络提供了新的见解。阻断ABA信号不影响乙烯诱导果实成熟的能力,而阻断可能抑制ABA促进果实成熟的有效性。这些结果表明ABA信号可能位于乙烯信号的上游调节果实成熟。我们的研究结果为植物激素在调节番茄果实成熟中的复杂调控网络提供了新的见解。阻断ABA信号不影响乙烯诱导果实成熟的能力,而阻断可能抑制ABA促进果实成熟的有效性。这些结果表明ABA信号可能位于乙烯信号的上游调节果实成熟。我们的研究结果为植物激素在调节番茄果实成熟中的复杂调控网络提供了新的见解。
更新日期:2022-06-07
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