A rapid, easy and simple method for the isolation and purification of α-glucosidase inhibitors of the ethyl acetate extract of Thymelaea hirsuta (EaTh) by a combination of thin layer chromatography (TLC) and enzymatic test has been developed. EaTh was demonstrated previously a potent α-glucosidase inhibitory effect. In this study, we developed a simple TLC-enzymatic test (TLC/EZ) combination to isolate α-glucosidase inhibitors present in EaTh.EaTh was extracted by Soxhlet from Thymelaea hirsuta (T. hirsuta). The EaTh was separated on a silica gel column and then on a TLC plate. After TLC separation, the TLC/EZ combination method was applied. α-glucosidase inhibitors were detected directly in the TLC plate using the glucose oxidase peroxidase method (GOD-POD). A good detection of active compounds was obtained in the TLC favoring the TLC/EZ method. Active compounds were then characterized using high performance liquid chromatography-mass spectrometry (HPLC–MS) analysis. The main α-glucosidase inhibitors present in EaTh have a molecular ion [M + H]+ at m/z = 543. This proposed method is suitable for a reliable isolation and purification of α-glucosidase inhibitors present in EaTh. It could be proposed as an interesting alternative of the classical method for the isolation and purification of α-glucosidase inhibitors in plant extracts.

中文翻译：

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从多毛瑞香中分离 α-葡萄糖苷酶抑制剂的薄层色谱-酶联用检测方法的开发

建立了薄层色谱(TLC)与酶法检测相结合的方法，快速、简便、简便地分离纯化多毛百里香(EaTh)乙酸乙酯提取物中的α-葡萄糖苷酶抑制剂。EaTh 先前已被证明具有有效的 α-葡萄糖苷酶抑制作用。在这项研究中，我们开发了一种简单的薄层色谱-酶促测试 (TLC/EZ) 组合来分离存在于 EaTh 中的 α-葡萄糖苷酶抑制剂。EaTh 是通过索氏萃取法从多毛百里香 (T. hirsuta) 中提取的。EaTh 在硅胶柱上分离，然后在 TLC 板上分离。TLC分离后，应用TLC/EZ联合方法。使用葡萄糖氧化酶过氧化物酶法 (GOD-POD) 直接在 TLC 板上检测 α-葡萄糖苷酶抑制剂。在有利于 TLC/EZ 方法的 TLC 中获得了对活性化合物的良好检测。然后使用高效液相色谱-质谱 (HPLC-MS) 分析对活性化合物进行表征。EaTh 中存在的主要 α-葡萄糖苷酶抑制剂在 m/z = 543 处具有分子离子 [M + H]+。该方法适用于可靠分离和纯化 EaTh 中存在的 α-葡萄糖苷酶抑制剂。它可以作为分离和纯化植物提取物中 α-葡萄糖苷酶抑制剂的经典方法的有趣替代方法。