As a research hotspot in the field of information processing, DNA computing exhibits several important underlying characteristics—from parallel computing and low energy consumption to high-performance storage capabilities—thereby enabling its wide application in nanomachines, molecular encryption, biological detection, medical diagnosis, etc. Based on DNA computing, the most rapidly developed field focuses on DNA molecular logic-gates computing. In particular, the recent advances in enzyme-based DNA logic gates has emerged as ideal materials for constructing DNA logic gates. In this review, we explore protein enzymes that can manipulate DNA, especially, nicking enzymes and polymerases with high efficiency and specificity, which are widely used in constructing DNA logic gates, as well as ribozyme that can construct DNA logic gates following various mechanism with distinct biomaterials. Accordingly, the review highlights the characteristics and applications of various types of DNAzyme-based logic gates models, considering their future developments in information, biomedicine, chemistry, and computers.

中文翻译：

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基于酶的DNA逻辑门的构建及应用研究进展


DNA计算作为信息处理领域的研究热点，展现出从并行计算、低能耗到高性能存储能力等几个重要的底层特性，从而使其在纳米机器、分子加密、生物检测、医疗诊断、以DNA计算为基础，发展最快的领域是DNA分子逻辑门计算。特别是，基于酶的 DNA 逻辑门的最新进展已成为构建 DNA 逻辑门的理想材料。在这篇综述中，我们探索了可以操纵DNA的蛋白质酶，特别是广泛用于构建DNA逻辑门的高效、特异性的切口酶和聚合酶，以及可以按照不同机制构建DNA逻辑门的核酶。生物材料。因此，考虑到它们在信息、生物医学、化学和计算机领域的未来发展，本文重点介绍了各种类型的基于 DNAzyme 的逻辑门模型的特征和应用。