Recently, a novel hybrid surface technology (HST) has been developed to mitigate metal analyte adsorption in liquid chromatography. The HST provides a hybrid organic–inorganic surface on the metal fluidic path, from injection to detector and including the column frits and wall, to mitigate the interaction between analytes and metals. Here the impact of the HST on the analysis of B group vitamins using liquid chromatography coupled with electrospray tandem mass spectrometry (LC-ESI-MS/MS) has been evaluated. Significant improvements in analyte intensity, limit of quantification (LOQ), carry-over, and peak shape were observed using an LC-ESI-MS/MS system and column that incorporated the HST. The key observed improvements include a 3–10 times increase in sensitivity (providing a lower LOQ) for riboflavin, thiamine, nicotinamide, FMN, PLP, and 5MTHF, no carry-over, and a more symmetrical peak for thiamine. When applied to the analysis of B group vitamins in energy drinks and B vitamin dietary supplement samples, the HST system demonstrated excellent accuracy and repeatability.

最近，已经开发出一种新型混合表面技术 (HST) 来减轻液相色谱中金属分析物的吸附。HST 在金属流路（从进样到检测器，包括柱筛板和柱壁）上提供有机-无机混合表面，以减轻分析物和金属之间的相互作用。此处评估了 HST 对使用液相色谱结合电喷雾串联质谱 (LC-ESI-MS/MS) 分析 B 族维生素的影响。使用包含 HST 的 LC-ESI-MS/MS 系统和色谱柱观察到分析物强度、定量限 (LOQ)、残留和峰形的显着改善。观察到的主要改进包括对核黄素、硫胺素、烟酰胺、FMN、PLP 和 5MTHF 的灵敏度提高 3-10 倍（提供更低的 LOQ），没有残留，硫胺素的峰更对称。当应用于分析能量饮料和 B 族维生素膳食补充剂样品中的 B 族维生素时，HST 系统表现出出色的准确性和可重复性。