Acceleration of lactate-utilizing pathway for enhancing biohydrogen production by magnetite supplementation in Clostridium butyricum,Bioresource Technology

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Acceleration of lactate-utilizing pathway for enhancing biohydrogen production by magnetite supplementation in Clostridium butyricum
Bioresource Technology ( IF 9.7 ) Pub Date : 2022-06-09 , DOI: 10.1016/j.biortech.2022.127448
Do-Hyung Kim ₁ , Jeong-Jun Yoon ₂ , Sang-Hyoun Kim ₃ , Jeong-Hoon Park ₄

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A conductive metal compound can be used as a catalyst for enhancing hydrogen production by dark fermentation. This study aimed to identify mechanisms of enhanced hydrogen production by magnetite supplementation. Experiments were performed with lactate and/or magnetite supplementation to confirm that the lactate-utilizing pathway is the key cause of enhanced hydrogen production. Also, ribonucleic acid sample was collected for monitoring gene regulation under each condition. Hydrogen production was significantly enhanced by approximately 25.6% and 58.9%, respectively, via magnetite alone and with lactate. Moreover, the expression of genes involved in hydrogen production, including pyruvate ferredoxin oxidoreductase, hydrogenase, and ferredoxin, via magnetite alone and with lactate was upregulated by 0.26, 0.71, and 3.50 and 1.06, 2.14, and 1.94 times, respectively.

中文翻译：

通过丁酸梭菌中补充磁铁矿加速乳酸利用途径以增强生物氢产量

导电金属化合物可用作提高暗发酵产氢的催化剂。本研究旨在确定通过补充磁铁矿提高氢气产量的机制。通过补充乳酸和/或磁铁矿进行实验，以确认乳酸利用途径是增强氢气产量的关键原因。此外，收集核糖核酸样品以监测每种条件下的基因调控。单独使用磁铁矿和使用乳酸时，氢气产量分别显着提高约 25.6% 和 58.9%。此外，单独使用磁铁矿和使用乳酸时，涉及氢气产生的基因（包括丙酮酸铁氧还蛋白氧化还原酶、氢化酶和铁氧还蛋白）的表达分别上调0.26、0.71和3.50倍以及1.06、2.14和1.94倍。

更新日期：2022-06-09

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