A conductive metal compound can be used as a catalyst for enhancing hydrogen production by dark fermentation. This study aimed to identify mechanisms of enhanced hydrogen production by magnetite supplementation. Experiments were performed with lactate and/or magnetite supplementation to confirm that the lactate-utilizing pathway is the key cause of enhanced hydrogen production. Also, ribonucleic acid sample was collected for monitoring gene regulation under each condition. Hydrogen production was significantly enhanced by approximately 25.6% and 58.9%, respectively, via magnetite alone and with lactate. Moreover, the expression of genes involved in hydrogen production, including pyruvate ferredoxin oxidoreductase, hydrogenase, and ferredoxin, via magnetite alone and with lactate was upregulated by 0.26, 0.71, and 3.50 and 1.06, 2.14, and 1.94 times, respectively.

导电金属化合物可用作催化剂，用于通过暗发酵提高产氢量。本研究旨在确定通过补充磁铁矿提高产氢的机制。用乳酸和/或磁铁矿补充剂进行了实验，以证实乳酸利用途径是提高氢气产量的关键原因。此外，收集核糖核酸样品用于监测每种条件下的基因调控。通过单独使用磁铁矿和使用乳酸，氢气产量分别显着提高了约 25.6% 和 58.9%。此外，单独通过磁铁矿和乳酸产生氢的相关基因（包括丙酮酸铁氧还蛋白氧化还原酶、氢化酶和铁氧还蛋白）的表达分别上调了 0.26、0.71 和 3.50 以及 1.06、2.14 和 1。