Transdifferentiation of mesenchymal stem cells (MSCs) into neurons provides a practical way for neurodegenerative diseases as alternatives to neural stem cells, but is confronted with challenges to get well-differentiated and mature neurons. In this work, a wirelessly triggered local electrical stimulation system was established to specifically induce neuronal differentiation from rat bone-marrow-derived MSCs (rBMSCs) by coupling a highly conductive and flexible multi-wall carbon nanotube (MWCNT) membrane with a rotating magnetic field. Without nerve-inducing factors, a nearly 100% yield of differentiated neurons was realised without the presence of astrocyte cells by the localized electrical stimuli mediated from electromagnetic induction nanogenerator. Neuronal functions were revealed by rapid spontaneous [Ca²⁺] i-transient peaks under neurotransmitter action. This novel therapeutic strategy for neurodegenerative disease was further demonstrated in vivo, where the successful neural differentiation of exogenous rBMSCs driven by external magnetic-filed accelerated the brain recovery. This wireless electric stimulation system shows promising effects on neuron differentiation and offers a new perspective in nerve repair without glial scarring.

间充质干细胞 (MSCs) 向神经元的转分化为神经退行性疾病提供了一种实用的方法，作为神经干细胞的替代品，但面临着获得分化良好和成熟的神经元的挑战。在这项工作中，建立了一种无线触发的局部电刺激系统，通过将高导电性和柔性多壁碳纳米管 (MWCNT) 膜与旋转磁场耦合，特异性诱导大鼠骨髓来源的 MSCs (rBMSCs) 的神经元分化。 . 在没有神经诱导因子的情况下，通过电磁感应纳米发电机介导的局部电刺激，在没有星形胶质细胞存在的情况下，实现了近 100% 的分化神经元产量。神经元功能通过快速自发 [Ca ²⁺] 神经递质作用下的 i 瞬态峰值。这种针对神经退行性疾病的新治疗策略在体内得到了进一步的证明，其中由外部磁场驱动的外源性 rBMSCs 的成功神经分化加速了大脑的恢复。这种无线电刺激系统显示出对神经元分化的良好影响，并为无胶质瘢痕的神经修复提供了新的视角。