Magnetic Particle Imaging of Magnetotactic Bacteria as Living Contrast Agents Is Improved by Altering Magnetosome Arrangement,Nano Letters

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Magnetic Particle Imaging of Magnetotactic Bacteria as Living Contrast Agents Is Improved by Altering Magnetosome Arrangement
Nano Letters ( IF 9.6 ) Pub Date : 2022-06-10 , DOI: 10.1021/acs.nanolett.1c05042
Ashley V Makela ₁ , Melissa A Schott ₁ , Cody S Madsen _{1,

2} , Emily M Greeson _{1,

3} , Christopher H Contag _{1,

2,

3}

Affiliation

Superparamagnetic iron oxide nanoparticles (SPIONs) can be used as imaging agents to differentiate between normal and diseased tissue or track cell movement. Magnetic particle imaging (MPI) detects the magnetic properties of SPIONs, providing quantitative and sensitive image data. MPI performance depends on the size, structure, and composition of nanoparticles. Magnetotactic bacteria produce magnetosomes with properties similar to those of synthetic nanoparticles, and these can be modified by mutating biosynthetic genes. The use of Magnetospirillum gryphiswaldense, MSR-1 with a mamJ deletion, containing clustered magnetosomes instead of typical linear chains, resulted in improved MPI signal and resolution. Bioluminescent MSR-1 with the mamJ deletion were administered into tumor-bearing and healthy mice. In vivo bioluminescence imaging revealed the viability of MSR-1, and MPI detected signals in livers and tumors. The development of living contrast agents offers opportunities for imaging and therapy with multimodality imaging guiding development of these agents by tracking the location, viability, and resulting biological effects.

中文翻译：

通过改变磁小体排列改善了作为活造影剂的趋磁细菌的磁粉成像

超顺磁性氧化铁纳米颗粒 (SPIONs) 可用作显像剂以区分正常组织和患病组织或跟踪细胞运动。磁粉成像 (MPI) 检测 SPION 的磁性，提供定量和敏感的图像数据。MPI 性能取决于纳米粒子的大小、结构和组成。趋磁细菌产生的磁小体具有与合成纳米颗粒相似的特性，并且可以通过使生物合成基因发生突变来对其进行修饰。使用带有mamJ缺失的Magnetospirillum gryphiswaldense 的 MSR-1 ，包含聚集的磁小体而不是典型的线性链，从而改善了 MPI 信号和分辨率。带有mamJ的生物发光 MSR-1对荷瘤小鼠和健康小鼠进行缺失。体内生物发光成像揭示了 MSR-1 的活力，MPI 在肝脏和肿瘤中检测到信号。活体造影剂的开发为成像和治疗提供了机会，多模态成像通过跟踪位置、活力和产生的生物效应来指导这些试剂的开发。

更新日期：2022-06-10

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