The English grain aphid (Sitobion avenae F.) causes significant yield and quality losses in wheat (Triticum aestivum L.) worldwide, and the use of insect-resistant wheat cultivars reduces the loss level. The present study aimed to identify quantitative trait loci (QTL) and perform putative candidate gene evolutionary analysis for English grain aphid resistance in 261 F_2:5 recombinant inbred lines (RILs) derived from the cross Amigo/XN1376. The RIL population and parents were genotyped with the Wheat 55K single nucleotide polymorphism (SNP) array. A total of 14,694 polymorphic SNPs were used to construct a genetic linkage map. One major effect of QTL was detected in F_2:5 RILs and was located on chromosome 3DL, explained 19.33% phenotypic variance, temporarily designated as QSa3.haust-3D. It was located in a 2.73 Mb, flanked by markers AX-109429351 and AX-110834100, from 550.25 to 552.98 Mb according to Chinese Spring reference genome. This region contained 39 annotated genes. Eight putative candidate genes for resistance to English grain aphid were identified among these genes. The QTL with closely linked SNP markers could be useful for marker-assisted selection for resistance to English grain aphid for wheat breeding.

中文翻译：

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普通小麦抗英国谷蚜位点的检测

英国谷物蚜虫 ( Sitobion avenae F.) 在全世界造成小麦 ( Triticum aestivum L.) 的显着产量和质量损失，使用抗虫小麦品种可降低损失水平。本研究旨在鉴定数量性状位点 (QTL) 并在源自杂交 Amigo/XN1376 的 261 F _2:5重组自交系 (RIL) 中对英国谷物蚜虫抗性进行推定的候选基因进化分析。使用小麦 55K 单核苷酸多态性 (SNP) 阵列对 RIL 种群和亲本进行基因分型。总共使用了 14,694 个多态性 SNP 来构建遗传连锁图谱。_{在 F 2:5}中检测到 QTL 的一个主要影响RILs位于染色体3DL，解释了19.33%的表型变异，暂命名为QSa3.haust-3D。它位于 2.73 Mb，两侧是标记AX-109429351和AX-110834100，根据 Chinese Spring 参考基因组，从 550.25 到 552.98 Mb。该区域包含 39 个注释基因。在这些基因中鉴定出八个假定的抗英国粮蚜的候选基因。具有紧密连锁的SNP标记的QTL可用于小麦育种抗英国谷蚜的标记辅助选择。