Single-cell transcriptomics reveals the natural product Shi-Bi-Man promotes hair regeneration by activating the FGF pathway in dermal papilla cells,Phytomedicine

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Single-cell transcriptomics reveals the natural product Shi-Bi-Man promotes hair regeneration by activating the FGF pathway in dermal papilla cells
Phytomedicine ( IF 6.7 ) Pub Date : 2022-06-09 , DOI: 10.1016/j.phymed.2022.154260
Mingrui Han ₁ , Chengxi Li ₂ , Chenyang Zhang ₁ , Chenglin Song ₁ , Qiang Xu ₃ , Qianqian Liu ₁ , Jianming Guo ₂ , Yang Sun ₃

Affiliation

Background

Finasteride and minoxidil are two commonly used drugs for the treatment of hair loss. However, these two drugs have certain side effects. Thus, the further elucidation of treatments for hair loss, including those using Chinese herbal medicine, remains important clinically. Shi-Bi-Man (SBM) is a hair health supplement that darkens hair and contains ginseng radix, tea polyphenols, polygonum multiflorum, radix angelicae sinensis, aloe, linseed, and green tea extract.

Purpose

This study aimed to find potential effective monomer components to promote hair regeneration from SBM and to explore the mechanism of SBM to promote hair regeneration.

Methods

Supplementation with the intragastric administration or smear administration of SBM in artificially shaved C57BL/6 mice, observe its hair growth. UPLC/MS and UPLC/LTQ-Orbitrap-MS detect the main components in SBM and the main monomers contained in the skin after smearing, respectively. A network pharmacology study on the main components of SBM and single-cell RNA sequencing was performed to explore the role of SBM for hair regeneration.

Results

SBM significantly induced hair growth compared with a control treatment. TSG and EGCG were the main monomers in the skin after SBM smearing. The results of single-cell sequencing revealed that after SBM treatment, the number of hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs) increased significantly. Cell interactions and volcano dots show that the interaction of the FGF signaling pathway was significantly enhanced, in which Fgf7 expression was especially upregulated in DPCs. In addition, the Wnt signaling pathway also had a partially enhanced effect on the interactions between various cells in the skin. The network pharmacology study showed that the promotion of the FGF and Wnt pathways by SBM was also enriched in alopecia diseases.

Conclusion

We report that SBM has a potential effect on the promotion of hair growth by mainly activating the FGF signaling pathway. The use of SBM may be a novel therapeutic option for hair loss.

中文翻译：

单细胞转录组学揭示天然产物 Shi-Bi-Man 通过激活真皮乳头细胞中的 FGF 通路促进头发再生

背景

非那雄胺和米诺地尔是治疗脱发的两种常用药物。但是，这两种药物都有一定的副作用。因此，进一步阐明脱发的治疗方法，包括使用中草药的治疗方法，在临床上仍然很重要。Shi-Bi-Man (SBM) 是一种头发保健品，可以使头发变黑，含有人参、茶多酚、何首乌、当归、芦荟、亚麻籽和绿茶提取物。

目的

本研究旨在从SBM中寻找促进毛发再生的潜在有效单体成分，并探索SBM促进毛发再生的机制。

方法

在人工剃毛的C57BL/6小鼠中补充SBM灌胃或涂片给药，观察其毛发生长情况。UPLC/MS 和 UPLC/LTQ-Orbitrap-MS 分别检测 SBM 中的主要成分和涂抹后皮肤中所含的主要单体。对 SBM 的主要成分和单细胞 RNA 测序进行了网络药理学研究，以探索 SBM 在毛发再生中的作用。

结果

与对照处理相比，SBM 显着诱导毛发生长。TSG和EGCG是SBM涂抹后皮肤中的主要单体。单细胞测序结果显示，经 SBM 处理后，毛囊干细胞 (HFSCs) 和真皮乳头细胞 (DPCs) 的数量显着增加。细胞相互作用和火山点表明FGF信号通路的相互作用显着增强，其中Fgf7的表达在DPCs中尤其上调。此外，Wnt 信号通路对皮肤中各种细胞之间的相互作用也有部分增强作用。网络药理学研究表明，SBM 对 FGF 和 Wnt 通路的促进作用也丰富了脱发疾病。