NADPH oxidases (NOXs) and hydrogen peroxide (H₂O₂) are involved in physiological and pathological processes, and cell fate decisions in organisms. However, regulatory mechanism of NOXs and the role of H₂O₂ on fungal sexual reproduction and host infection remain largely unexplored. Here, we identified ROS metabolic genes and key signalling genes of MAPK and cAMP-PKA pathways in Curvularia lunata, which were NOX ClNOX1 and ClNOXR, superoxide dismutase ClSOD1 and catalase ClCAT4, redox-regulated transcription factor ClAP1, Ras small GTPases Clg2P, pheromone-response MAPK ClK1 and cAMP-PKA ClSCHA, and characterized the functions of these genes. The results showed that ClNOX1 localized to the plasma membrane. ClNOX1 and ClNOXR were involved in sexual reproduction and host infection via ClNOX1/ClNOXR-derived H₂O₂ as well as MAPK and cAMP-PKA signalling pathways. H₂O₂ acted as a signalling molecule to regulate sexual reproduction and host infection in C. lunata.

中文翻译：

---

ClNOX1/ClNOXR 介导的 MAPK 和 cAMP-PKA 信号通路和 ROS 代谢参与弯弯孢子有性繁殖和宿主感染

NADPH 氧化酶 (NOX) 和过氧化氢 (H ₂ O ₂ ) 参与生物体的生理和病理过程以及细胞命运决定。然而，NOXs 的调控机制和 H ₂ O ₂对真菌有性生殖和宿主感染的作用在很大程度上仍未得到探索。在这里，我们确定了弯弯菌中 MAPK 和 cAMP-PKA 通路的 ROS 代谢基因和关键信号基因，即 NOX ClNOX1和ClNOXR、超氧化物歧化酶ClSOD1和过氧化氢酶ClCAT4、氧化还原调节转录因子ClAP1、Ras 小 GTPase Clg2P, 信息素反应 MAPK ClK1和 cAMP-PKA ClSCHA , 并表征了这些基因的功能。结果显示ClNOX1定位于质膜。ClNOX1和ClNOXR通过ClNOX1 / ClNOXR衍生的H ₂ O ₂以及MAPK和cAMP-PKA信号通路参与有性生殖和宿主感染。H ₂ O ₂作为信号分子调节C中的有性生殖和宿主感染。新月_