In this study, the GC/PEI/CNT/S.p./GOx bioanode was successfully designed using the chemical oxidized multi-walled carbon nanotubes (CNT) and Spirulina platensis-based lysates that facilitate the electron transfer and reduce the open circuit potential (OCP) drop along the electron transfer pathway. Composition, morphology and chemical modification efficiency of CNT was examined using scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX) and simultaneous thermal analysis (STA) coupled with mass spectrometric (MS) analysis of evolving gaseous species (TG/DTA–MS). The power density generated by GC/PEI/CNT/S.p./GOx bioelectrode was 21.8 times higher if S. platensis-based lysates were applied in bioanode design (than GC/PEI/CNT/GOx bioelectrode). The magnitude of maximal power density evaluated by linear sweep voltammetry (LSV) measurements was estimated to be 3.2 µW cm⁻² at 213 mV voltage, whereas the bioelectrode act as a bioanode and bare glassy carbon as a cathode in a single-compartment biofuel cell design. Furthermore, the GC/PEI/CNT/S.p./GOx bioanode shows high long-term stability, while the magnitude of power density after seven days achieved approximately 89.6 % (2.87 µW cm⁻²) of its initial value. The initial OCP value of bioanode with S. platensis-based lysates was found to be -156 mV, which was reduced after the addition of 12.5 mM glucose. The equilibrated average value of OCP (-259 mV vs Ag/AgCl (3 M KCl)) indicates that glucose oxidase (GOx) immobilized on CNT functionalized with S. platensis-based lysates possess superior electron transfer and reduce the OCP drop along the electron transfer pathway.

在这项研究中，GC/PEI/CNT/Sp/GOx 生物阳极是使用化学氧化多壁碳纳米管 (CNT) 和基于螺旋藻的裂解物成功设计的，这些裂解物有助于电子转移并降低开路电位 (OCP)沿电子转移路径下降。使用扫描电子显微镜 (SEM)、能量色散 X 射线分析 (EDX) 和同时热分析 (STA) 结合质谱 (MS) 分析演化的气体物质 (TG/ DTA-MS）。如果 S. platensis GC/PEI/CNT/Sp/GOx 生物电极产生的功率密度是S. platensis的 21.8 倍基于 - 的裂解物应用于生物阳极设计（而不是 GC/PEI/CNT/GOx 生物电极）。在 213 mV 电压下，通过线性扫描伏安法 (LSV) 测量评估的最大功率密度幅度估计为 3.2 µW cm ^-2，而在单室生物燃料电池中，生物电极充当生物阳极，裸玻璃碳充当阴极设计。此外，GC/PEI/CNT/Sp/GOx 生物阳极显示出较高的长期稳定性，而 7 天后的功率密度幅度达到其初始值的约 89.6 % (2.87 µW cm ^-2 )。发现具有基于S. platensis的裂解物的生物阳极的初始 OCP 值为-156 mV，在添加 12.5 mM 葡萄糖后降低。OCP 的平衡平均值 (-259 mV vsAg/AgCl (3 M KCl)) 表明固定在用基于S. platensis的裂解物功能化的 CNT 上的葡萄糖氧化酶 (GOx) 具有优异的电子转移并减少沿电子转移途径的 OCP 下降。