In this study, microwave pretreatment and grinding treatment were used to enhance sulforaphane formation, then ultrasonic-assisted extraction (UAE) was applied to extract sulforaphane using simultaneous hydrolysis and extraction method. The effects of various parameters, which were ultrasonic time, ultrasonic power, solid-water ratio and solid-ethyl acetate ratio on the extraction rate of sulforaphane were investigated. The results showed that microwave pretreatment enhanced sulforaphane formation. Excessive size reduction did not increase or even reduced extraction rate of sulforaphane. Simultaneous hydrolysis and extraction significantly increased extraction rate of sulforaphane compared to hydrolysis followed by extraction. UAE accelerated mass transfer and the solubilization of the targeted compounds due to the acoustic cavitation effect, thus enhanced enzymatic hydrolysis of glucoraphanin and the extraction rate of sulforaphane. The extraction rate of sulforaphane using UAE with simultaneous hydrolysis and extraction was 4.07-fold of the conventional extraction method. UAE was an effective method to extract sulforaphane from broccoli seeds since it led to higher yield of sulforaphane in a much shorter extraction time.

本研究采用微波预处理和研磨处理来促进萝卜硫素的形成，然后采用超声波辅助萃取（UAE）采用同时水解和萃取的方法提取萝卜硫素。各种参数的影响，如超声波时间， 考察了超声功率、固水比和固-乙酸乙酯比对萝卜硫素提取率的影响。结果表明，微波预处理增强了萝卜硫素的形成。过度减小尺寸不会增加或什至降低萝卜硫素的提取率。与水解后提取相比，同时水解和提取显着提高了萝卜硫素的提取率。由于声空化效应，UAE加速了传质和目标化合物的溶解，从而提高了萝卜硫素的酶解和萝卜硫素的提取率。使用UAE同时水解和提取的萝卜硫素提取率为常规提取方法的4.07倍。