Abstract A rapid, simple and economical spectrofluorimetric method for the determination of diclofenac potassium in pure form, in pharmaceutical preparations and in human plasma has been developed. The method is based on the enhancement of the fluorescence signal of diclofenac potassium by the addition of sodium dodecyl sulphate in McIvaine buffer with a pH of 5. Different experimental conditions such as buffer type, pH, type and concentration of surfactants were investigated. The fluorescence intensity of the solution was recorded at 361 nm after excitation at 243 nm. The method shows linearity in the concentration range of 0.2 μg mL–1–10 μg mL–1 with a good correlation coefficient of 0.997. The relative standard deviation value was 3.62 (n = 7). The limit of detection and limit of quantification were calculated to be 2.84 × 10–3 μg mL–1 and 9.47 × 10–3 μg mL-1, respectively. The effect of excipients and co-administrated drugs was investigated and no interference was observed. The method was successfully applied for the determination of diclofenac potassium in pure form, in pharmaceutical products and in human plasma. The percentage recoveries obtained ranged from 100.25% to 102.16% for pure form and 97.50% to 102.00% for pharmaceutical products and from 98.50% to 101.67% for human plasma.

中文翻译：

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胶束增强荧光光谱法定量双氯芬酸钾纯品，药物制剂和人血浆

摘要 建立了一种快速、简单、经济的荧光光谱法，用于测定纯品、药物制剂和人血浆中的双氯芬酸钾。该方法基于通过在 pH 值为 5 的 McIvaine 缓冲液中添加十二烷基硫酸钠来增强双氯芬酸钾的荧光信号。研究了不同的实验条件，如缓冲液类型、pH、表面活性剂的类型和浓度。在 243 nm 激发后，在 361 nm 记录溶液的荧光强度。该方法在 0.2 μg mL-1-10 μg mL-1 的浓度范围内显示出线性，良好的相关系数为 0.997。相对标准偏差值为 3.62 (n = 7)。检测限和定量限计算为2。分别为 84 × 10-3 μg mL-1 和 9.47 × 10-3 μg mL-1。研究了赋形剂和共同给药药物的影响，没有观察到干扰。该方法成功地用于测定纯品、药品和人血浆中的双氯芬酸钾。获得的回收百分比范围为纯形式的 100.25% 至 102.16%，医药产品的 97.50% 至 102.00%，以及人血浆的 98.50% 至 101.67%。