Breast cancer is the most common type of cancer among women. Radiotherapy is one of the treatment options for breast cancer; it plays a significant role in tumour control after surgery. However, radio resistance developed by tumour cells is the main problem in radiotherapy. As such, radiosensitization agents are used to improve radiotherapy efficiency. This study aims to investigate the role of octreotide acetate as a radiosensitizer for MCF7 cells. The treatments were designated into four groups: untreated cells, cells treated with octreotide acetate, cells treated with X-ray and cells treated with octreotide acetate in combination with X-ray irradiation. Cell viability assay, clonogenic assay, and qPCR was used as BAX apoptosis indicator marker analysis Morphological changes were examined under an inverted microscope. The results showed more effectiveness for the octreotide acetate combination to X-ray radiation in the MCF7 cell line. These findings suggested that octreotide acetate enhanced radiosensitivity and induced apoptosis in MCF7 cell line.

中文翻译：

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醋酸奥曲肽增强 MCF7 乳腺癌细胞系的放射敏感性并诱导细胞凋亡

乳腺癌是女性中最常见的癌症类型。放射治疗是乳腺癌的治疗选择之一；它在手术后的肿瘤控制中发挥着重要作用。然而，肿瘤细胞产生的放射抗性是放射治疗的主要问题。因此，放射增敏剂用于提高放射治疗效率。本研究旨在探讨醋酸奥曲肽作为 MCF7 细胞放射增敏剂的作用。将处理分为四组：未处理的细胞、用醋酸奥曲肽处理的细胞、用X射线处理的细胞以及用醋酸奥曲肽结合X射线照射处理的细胞。采用细胞活力测定、克隆形成测定和qPCR作为BAX凋亡指示标记分析，在倒置显微镜下观察形态变化。结果表明，醋酸奥曲肽组合对 MCF7 细胞系中的 X 射线辐射更有效。这些发现表明，醋酸奥曲肽增强了 MCF7 细胞系的放射敏感性并诱导细胞凋亡。