Multimodal strategies of cancer treatment such as chemoradiotherapy aim to eradicate complex malignant diseases with enhanced therapeutic outcome. This study aimed to investigate the effects of bismuth oxide nanoparticles (BiONPs) and cisplatin (Cis) (BC) under gamma irradiation from Ir-192 of clinical High Dose Rate (HDR) brachytherapy. The cells irradiations were performed using 0.38 MeV Iridium-192 HDR Brachytherapy source with doses of 0–4 Gy in a single fraction. Apoptosis analyses were conducted using flow cytometry and subcellular biochemical changes were evaluated using Raman spectroscopy. The percentage of apoptosis of MCF-7 breast cancer cells with BC indicated an increment with early apoptosis were more predominant compared to late apoptotic cells. The cells population profile also shown cells shrinkage and cellular membrane damages for BC treated cells after brachytherapy. Subcellular biochemical changes were detected which involved the glycolysis process, the disarrangement of the amino acid structure and DNA/RNA disintegration that might be associated with cells death mechanism. This study demonstrated that the BiONPs-Cis (BC) can potentially enhanced radiation effects of brachytherapy due to the potent synergetic effects that will progressively enhance the therapeutic impact of cancer treatment.

中文翻译：

---

氧化铋纳米颗粒和顺铂对 Ir-192 高剂量率近距离放射治疗 MCF-7 乳腺癌细胞的影响

癌症治疗的多模式策略（例如放化疗）旨在根除复杂的恶性疾病并增强治疗效果。本研究旨在探讨临床高剂量率 (HDR) 近距离放射治疗中 Ir-192 伽马射线照射下氧化铋纳米颗粒 (BiONPs) 和顺铂 (Cis) (BC) 的效果。使用 0.38 MeV Iridium-192 HDR 近距离放射治疗源进行细胞照射，单次剂量为 0-4 Gy。使用流式细胞术进行细胞凋亡分析，并使用拉曼光谱评估亚细胞生化变化。具有 BC 的 MCF-7 乳腺癌细胞的凋亡百分比表明，与晚期凋亡细胞相比，早期凋亡的增加更为显着。细胞群体概况还显示了近距离放射治疗后 BC 处理的细胞的细胞收缩和细胞膜损伤。检测到的亚细胞生化变化涉及糖酵解过程、氨基酸结构的紊乱和 DNA/RNA 分解，这些可能与细胞死亡机制有关。这项研究表明，BiONPs-Cis (BC) 可能会增强近距离放射治疗的放射效果，因为其强大的协同效应将逐步增强癌症治疗的治疗效果。