Abstract Studies on the chemical modifications of Rosmarinus officinalis essential oil hydrodistillation process (HD) by using 5% citric acid (CA-HD) and 5% trisodium citrate (TSC-HD) as a water phase were performed. Composition of essential oils obtained in conventional and modified conditions was analyzed by gas chromatography with mass selective detector method (GC-MS) and compared. Antioxidant activity of all essential oils was determined spectrophotometrically by using DPPH radical scavenging method. It was found that applied modifications of hydrodistillation process enhanced yields and antioxidant activity and the best results were obtained using 5% citric acid as a modifier. Effect of this modification on fungicidal activity of essential oils against 8 various fungi strains (Alternaria alternata, Botrytis cinerea, Fusarium culmorum, Phythophtora cactorum, Rhizoctonia solani, Phythophtora infestans, Sclerotinia sclerotiorum and Ascosphaera apis) was also determined and in most cases enhanced activity was observed.

中文翻译：

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加氢蒸馏化学改性对迷迭香精油产量、组成及生物活性的影响

摘要 以5%柠檬酸（CA-HD）和5%柠檬酸三钠（TSC-HD）为水相对迷迭香精油加氢蒸馏工艺（HD）进行了化学改性研究。通过气相色谱和质量选择检测器法 (GC-MS) 分析在常规条件和改进条件下获得的精油成分，并进行比较。采用DPPH自由基清除法分光光度法测定所有精油的抗氧化活性。发现加氢蒸馏工艺的应用改进提高了收率和抗氧化活性，并且使用 5% 柠檬酸作为改性剂获得了最佳结果。这种修饰对精油对 8 种不同真菌菌株（Alternaria alternata、Botrytis cinerea、