Abstract Plant-esterase (EC 3.1.1.X) has received much attention because plant esterase and acetylcholinesterase (AChE) share a similar sensitivity towards organophosphorus (OP) pesticides detection with the same inhibition mechanism. To improve the analytical performance, tetraphenyl metal porphyrin, as an indicator was introduced to combine with plant-esterase. The time of reach equilibrium in PBS solution was shortened after adding plant-esterase by assaying the intensify change of the porphyrin spectrum. Meanwhile, intensify of porphyrin spectrum with plant-esterase was increased compared with that of only the porphyrin spectrum in solution. Tetraphenyl metal porphyrin, such as cobalt(II) meso-tetraphenyl porphyrin, is a mixed reversible inhibitor of plant-esterase from kinetic parameters. The combination ratio of plant-esterase and porphyrin is 2:1. On the other hand, the interaction between CoTPPCl and plant-esterase is the strongest among all tested tetraphenyl metal porphyrin. And the mixed system (CoTPPCl-plant-esterase) showed the best sensitivity towards the tested pesticide. All these results indicated that a complex system composed of tetraphenyl metal porphyrin and plant-esterase was fit for detecting pesticides. They make meaningful guidance on the further design of sensing material in monitoring pesticides.

中文翻译：

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确定钴（II）内消旋四苯基卟啉与植物酯酶的结合机制

摘要 植物酯酶 (EC 3.1.1.X) 受到广泛关注，因为植物酯酶和乙酰胆碱酯酶 (AChE) 对有机磷 (OP) 农药的检测具有相似的敏感性，具有相同的抑制机制。为提高分析性能，引入了作为指示剂的四苯基金属卟啉与植物酯酶结合。通过测定卟啉光谱的剧烈变化，加入植物酯酶后，PBS溶液达到平衡的时间缩短。同时，植物酯酶对卟啉谱的增强与溶液中仅卟啉谱的增强相比有所增加。四苯基金属卟啉，如钴 (II) 内消旋四苯基卟啉，是一种从动力学参数上看的植物酯酶的混合可逆抑制剂。植物酯酶与卟啉的组合比例为2:1。另一方面，CoTPPCl与植物酯酶的相互作用在所有测试的四苯基金属卟啉中最强。并且混合系统（CoTPPCl-植物-酯酶）对被测农药表现出最好的敏感性。所有这些结果表明，由四苯基金属卟啉和植物酯酶组成的复杂体系适合用于农药检测。他们为进一步设计用于监测农药的传感材料提供了有意义的指导。所有这些结果表明，由四苯基金属卟啉和植物酯酶组成的复杂体系适合用于农药检测。他们为进一步设计用于监测农药的传感材料提供了有意义的指导。所有这些结果表明，由四苯基金属卟啉和植物酯酶组成的复杂体系适合用于农药检测。他们为进一步设计用于监测农药的传感材料提供了有意义的指导。