Abstract Asthma, the respiratory disorder associated with bronchial hyper-responsiveness, affected 300 million people across the globe, with a prevalence of 4.05% in Saudi Arabia and causing 61.6% of hospital emergency room annual visits. Increased side effects of conventional drugs demand the necessity for the development of natural drugs. In this study, an herbal composite from Bacopa monnieri and Euphorbia hirta was prepared and characterized by Fourier transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) analysis. In vitro bacterial inhibition and anti-asthmatic activity were evaluated using animal models. Ethanolic herbal composite (EHC) showed significant anti-pathogenic activities. GC-MS analysis identified potential bioactive compounds and FT-IR analysis revealed functional groups corresponding to plant composites. The EHC increased the preconvulsive time against 1% histamine aerosol compared to control animals. In sensitized + EHC-treated animals, total leukocyte, eosinophil, lymphocyte, neutrophil, and monocyte counts were found to be reduced as compared to sensitized and control groups. EHC decreased malondialdehyde and bicarbonate levels denoting the reduced oxidative burden and increased the antioxidant activity by increased intracellular glutathione (GSH) level. The EHC-treated group showed decreased inflammatory cell infiltration compared to the sensitized. A significant anti-asthmatic effect was observed in the EHC-treated group (P < 0.05). Thus, herbal composites are used in the treatment of asthma and can be used as an alternative to commercially available pharmaceutical drugs.

中文翻译：

---

来自植物假马齿苋和大戟属植物的沙特草药复合物对豚鼠的抗哮喘活性

摘要哮喘是一种与支气管高反应性相关的呼吸系统疾病，在全球影响了 3 亿人，在沙特阿拉伯的患病率为 4.05%，每年占医院急诊室就诊人数的 61.6%。常规药物副作用增加，需要开发天然药物。在这项研究中，制备了一种来自假马齿苋和大戟的草药复合物，并通过傅里叶变换红外光谱 (FT-IR) 和气相色谱-质谱 (GC-MS) 分析对其进行了表征。使用动物模型评估体外细菌抑制和抗哮喘活性。乙醇草本复合物 (EHC) 显示出显着的抗病原活性。GC-MS 分析确定了潜在的生物活性化合物，FT-IR 分析揭示了与植物复合材料相对应的官能团。与对照动物相比，EHC 增加了针对 1% 组胺气溶胶的惊厥前时间。在致敏 + EHC 治疗的动物中，与致敏组和对照组相比，发现总白细胞、嗜酸性粒细胞、淋巴细胞、中性粒细胞和单核细胞计数减少。EHC 降低了丙二醛和碳酸氢盐水平，表明氧化负荷降低，并通过增加细胞内谷胱甘肽 (GSH) 水平来增加抗氧化活性。与致敏组相比，EHC 治疗组显示炎症细胞浸润减少。在 EHC 治疗组中观察到显着的抗哮喘作用（P < 0.05）。因此，