Abstract Plant-mediated silver nanoparticles are unique and are considered one of the best nanomaterials used in cancer research. We report a low-cost, eco-friendly process of green synthesis of AgNPs from Commiphora gileadensis stem extracts and evaluated their anticancer potential against colon cancer cell lines HCT-116, HT 29, and SW620. Anticancer activities were performed by an MTT assay and gene expression levels of four genes CHEK1, CHEK2, ATR, and ATM by the real-time polymerase chain reaction. Particles were initially confirmed by UV-visible spectroscopy. The morphology and stability of the particles were examined through TEM, zeta potential, and zeta sizer. GC-MS and FTIR were performed to examine the functional groups. The absorption peak was recorded at 430 nm; the average size recorded by TEM images was 13 nm, while the zeta potential and zeta sizer study showed aggregation in nanoparticles. Compared to C. gileadensis extracts, some of the FT-IR spectrum peaks were sight shifted with some new peaks in C. gileadensis AgNPs. C. gileadensis AgNPs were more toxic against HT29 followed by HTC116 and SW620. Expression levels of most of the genes in HCT116 and HT29 were increased by treatment whereas the gene expression level was least affected in SW620. C. gileadensis AgNPs have anticancer potential and need to be explored in cancer research.

中文翻译：

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使用吉列无花果干提取物对人结肠癌细胞的生物源银纳米粒子的抗癌潜力

摘要 植物介导的银纳米粒子是独一无二的，被认为是癌症研究中使用的最佳纳米材料之一。我们报告了一种低成本、环保的从 Commiphora gileadensis 茎提取物绿色合成 AgNPs 的过程，并评估了它们对结肠癌细胞系 HCT-116、HT 29 和 SW620 的抗癌潜力。抗癌活性通过 MTT 测定和四个基因 CHEK1、CHEK2、ATR 和 ATM 的基因表达水平通过实时聚合酶链反应进行。粒子最初是通过紫外-可见光谱确认的。通过 TEM、zeta 电位和 zeta sizer 检测颗粒的形态和稳定性。进行 GC-MS 和 FTIR 以检查官能团。在 430 nm 处记录吸收峰；TEM 图像记录的平均尺寸为 13 nm，而 zeta 电位和 zeta sizer 研究显示纳米粒子聚集。与 C. gileadensis 提取物相比，一些 FT-IR 光谱峰在 C. gileadensis AgNPs 中出现了一些新峰的视线偏移。C. gileadensis AgNPs 对 HT29 的毒性更大，其次是 HTC116 和 SW620。HCT116 和 HT29 中大多数基因的表达水平通过处理增加，而 SW620 中基因表达水平的影响最小。C. gileadensis AgNPs 具有抗癌潜力，需要在癌症研究中进行探索。HCT116 和 HT29 中大多数基因的表达水平通过处理增加，而 SW620 中基因表达水平的影响最小。C. gileadensis AgNPs 具有抗癌潜力，需要在癌症研究中进行探索。HCT116 和 HT29 中大多数基因的表达水平通过处理增加，而 SW620 中基因表达水平的影响最小。C. gileadensis AgNPs 具有抗癌潜力，需要在癌症研究中进行探索。