Introduction:Iris taochia is an endemic plant in Turkey. Iris species has many biologicaleffects such as antibacterial, antiinflammatory, antioxidant and anticancer properties. Apoptosisis a programmed cell death and this mechanism regulates the death of cancer cells.Purpose:The aim of our work is to investigate how the Iris taochia extracts affect the apoptotic activityin the MCF7 cells.Methods:Cytotoxic dose and cell viability is determined by the MTT assay. Bad, Bax, Bcl-2, Bcl-W, Bid, Bim, Caspase 3, Caspase 8, CD40, CD40L, cIAP-2, CytoC, DR6, Fas, FasL, HSP27,HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5,IGFBP-6, IGF-1sR, Livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-α, TNF-β,TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4 and XIAP proteins were measured by the membranearray kit.Results:Iris taochia extracts exhibited significant cytotoxic effects on MCF7 cells and IC50 valuesranging from 1.56 to 100 μg/mL. Our results indicate that MeOH extract of Iris taochia in MCF7cells may be a regulator of cell death proteins, cell cycle and growth factors. DCM and EtOH extractsof Iris taochia have a limited effect on MCF7 cells, especially, HSPs, which play a significantrole in chemoresistance, downregulating DCM and EtOH extracts of Iris taochia, whereas ligandsand receptors of extrinsic apoptotic pathway are upregulated by these extracts.Conclusion:This is the first study to investigate the cytotoxic and apoptotic effect of Iris taochiaextracts on MCF7 cells. Results also showed that Iris taochia reduced cell viability and inducedapoptotic pathways as a potential regulator of cancer cell death.

中文翻译：

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桃子鸢尾植物提取物对人乳腺癌（MCF-7）细胞的细胞毒和凋亡作用

简介：桃花鸢尾是土耳其特有的植物。鸢尾属植物具有许多生物效应，例如抗菌、抗炎、抗氧化和抗癌特性。细胞凋亡是一种程序性细胞死亡，这种机制调节癌细胞的死亡。目的：我们的工作目的是研究鸢尾花提取物如何影响 MCF7 细胞的凋亡活性。方法：细胞毒性剂量和细胞活力由 MTT 确定化验。坏的，Bax，Bcl-2，Bcl-W，Bid，Bim，Caspase 3，Caspase 8，CD40，CD40L，cIAP-2，CytoC，DR6，Fas，FasL，HSP27，HSP60，HSP70，HTRA，IGF-I， IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5,IGFBP-6, IGF-1sR, Livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-膜阵列试剂盒检测R2、TNF-α、TNF-β、TRAILR-1、TRAILR-2、TRAILR-3、TRAILR-4和XIAP蛋白。 结果：桃子鸢尾提取物对 MCF7 细胞具有显着的细胞毒作用，IC50 值范围为 1.56 至 100 μg/mL。我们的结果表明，MCF7细胞中桃子鸢尾的MeOH提取物可能是细胞死亡蛋白、细胞周期和生长因子的调节剂。桃子鸢尾DCM和EtOH提取物对MCF7细胞的作用有限，尤其是HSPs，其在化学抗性中起重要作用，下调桃子鸢尾DCM和EtOH提取物，而这些提取物上调外源性凋亡途径的配体和受体。结论：这是第一项研究鸢尾花提取物对 MCF7 细胞的细胞毒性和凋亡作用的研究。结果还表明，桃子鸢尾作为癌细胞死亡的潜在调节剂可降低细胞活力并诱导细胞凋亡途径。