Background: In type 2 diabetic mouse liver, hyperglycemia, and insulin modify gene expression. Curcumin is apowerful antioxidant and antidiabetic agent that regulates the gene expression of different signaling pathways throughvarious transcription factors. Therefore, we hypothesized that curcumin modifies the protein expression profile in the liverof diabetic db/db mice.Objective:To determine the effects of curcumin on the liver protein profile of diabetic db/db mice.Methods:db/db and wild type (WT) male mice were allocated in four groups, and they were fed for eight weeks. Three WTand three diabetic db/db mice received a standard diet (SD; WT and db/db groups, respectively); three WT and threediabetic db/db mice received a SD supplemented with 0.75 % (w/w) curcumin (WT+C and db/db+C groups, respectively).Liver proteins were separated by 2D electrophoresis. Differential protein expression analysis was performed onImageMaster 2D Platinum software, and selected proteins were identified by MALDI-TOF-MS and subjected to enrichmentanalysis using STRING and DAVID databases.Results:Thirty-six proteins with differential expression due to the diabetic background and curcumin treatment were found;these proteins participate in the metabolism of amino acids, carbohydrates, and lipids. Interestingly, the altered expression ofseven proteins was prevented in the liver of the diabetic mice that received curcumin.Conclusions:Among all differentially expressed proteins, curcumin reverted the altered expression of seven proteins. Thus,although it was observed that curcumin did not affect the biochemical parameters, it does modify the expression of someliver proteins in diabetic mice.

中文翻译：

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姜黄素可逆转糖尿病肥胖 db/db 小鼠肝脏中的蛋白质差异表达

背景：在 2 型糖尿病小鼠肝脏中，高血糖和胰岛素会改变基因表达。姜黄素是一种强大的抗氧化剂和抗糖尿病药物，通过各种转录因子调节不同信号通路的基因表达。因此，我们假设姜黄素改变了糖尿病db/db小鼠肝脏中的蛋白质表达谱。目的：确定姜黄素对糖尿病db/db小鼠肝脏蛋白谱的影响。方法：db/db和野生型（WT ) 雄性小鼠被分成四组，喂食八周。三只 WT 和三只糖尿病 db/db 小鼠接受标准饮食（SD；分别为 WT 和 db/db 组）；三只 WT 和三只糖尿病 db/db 小鼠接受补充有 0.75% (w/w) 姜黄素的 SD（分别为 WT+C 和 db/db+C 组）。通过二维电泳分离肝蛋白。在 ImageMaster 2D Platinum 软件上进行差异蛋白表达分析，并通过 MALDI-TOF-MS 鉴定选定的蛋白质，并使用 STRING 和 DAVID 数据库进行富集分析。结果：36 种蛋白质因糖尿病背景和姜黄素治疗而出现差异表达。发现；这些蛋白质参与氨基酸、碳水化合物和脂质的代谢。有趣的是，在接受姜黄素治疗的糖尿病小鼠的肝脏中，七种蛋白质的表达改变被阻止了。结论：在所有差异表达的蛋白质中，姜黄素恢复了七种蛋白质的改变表达。因此，虽然观察到姜黄素不影响生化参数，