Copper metal organic framework as natural oxidase mimic for effective killing of Gram-negative and Gram-positive bacteria,Nanoscale

当前位置： X-MOL 学术 › Nanoscale › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Copper metal organic framework as natural oxidase mimic for effective killing of Gram-negative and Gram-positive bacteria
Nanoscale ( IF 5.8 ) Pub Date : 2022-06-08 , DOI: 10.1039/d2nr01673g
Zhihui Mao _{1,

2} , Jie Chen _{2,

3} , Yindian Wang ₂ , Junjie Xia ₂ , Yajing Zhang ₄ , Weiwen Zhang ₄ , Han Zhu ₂ , Xiaojun Hu ₂ , Hongxia Chen ₂

Affiliation

Nanozymes have been widely studied as substitutes for natural enzymes. However, the delicacy of their structures and their unclear catalytic sites make it difficult to maintain their structural robustness and catalytic durability. By mimicking active catalytic sites of natural enzymes and combining them with distinct channels of metal organic frameworks (MOFs), an active copper mimetic oxidase enzyme (Cu-MOF) was designed and synthesized with good structure and clear catalytic sites for improvement in catalytic activity. The Cu-MOFs showed excellent oxidase-like activity with a low K_m of 1.09 mM and exogenous ROS generation capacity. The Cu-MOFs exhibited antibacterial efficacy at a low concentration of 12.5 μg mL⁻¹ by an oxidative stress response. These Cu-MOFs with their simple design and effective oxidase mimicking show attractive application prospects in the field of antibacterial and enzyme catalysis.

中文翻译：

铜金属有机骨架作为天然氧化酶模拟物有效杀死革兰氏阴性和革兰氏阳性细菌

纳米酶已被广泛研究作为天然酶的替代品。然而，它们结构的微妙性和不明确的催化位点使其难以保持其结构稳健性和催化耐久性。通过模拟天然酶的活性催化位点，并将其与金属有机框架（MOFs）的不同通道相结合，设计合成了一种结构良好、催化位点清晰的活性铜模拟氧化酶（Cu-MOF），以提高催化活性。Cu-MOFs 表现出优异的类氧化酶活性，具有1.09 mM的低K _{m和外源 ROS 生成能力。}^{Cu-MOFs 在 12.5 μg mL -1}的低浓度下表现出抗菌功效通过氧化应激反应。这些Cu-MOFs以其简单的设计和有效的氧化酶模拟在抗菌和酶催化领域显示出诱人的应用前景。

更新日期：2022-06-08

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11