An activatable near-infrared hemicyanine-based probe for selective detection and imaging of Hg2+ in living cells and animals,Analyst

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An activatable near-infrared hemicyanine-based probe for selective detection and imaging of Hg2+ in living cells and animals
Analyst ( IF 3.6 ) Pub Date : 2022-06-07 , DOI: 10.1039/d2an00125j
Min Li ₁ , Ruiyu Li ₁ , Xuefei Chen ₁ , Jin Liu ₁ , Yong Shao ₂ , Zhiai Xu ₁ , Wen Zhang ₁

Affiliation

Fluorescence imaging in the near-infrared (NIR) window has great potential for clinical diagnosis and treatment because of its deep penetration and high contrast. Here, a NIR hemicyanine-based probe (CyP) was synthesized for selective detection and imaging of Hg²⁺ in living cells and animals. Using the rigid xanthene structure as the electron donor, trimethylindolenine as the electron acceptor and diphenylphosphinothioic chloride as the recognition element, the probe CyP could specifically respond to Hg²⁺ and transform into an activated donor–π-acceptor (D–π-A) motif with a NIR emission maximum at 710 nm. Cell and animal imaging experiments showed that the probe CyP could be activated by Hg²⁺ and had good concentration dependence in imaging. Meanwhile, animal imaging experiments showed that the activated CyP probe exhibited a higher tissue penetration depth and spatiotemporal resolution. Thus, the probe CyP could be a useful tool for monitoring and visually evaluating Hg²⁺ in living systems.

中文翻译：

一种可激活的基于近红外半花青的探针，用于对活细胞和动物中的 Hg2+ 进行选择性检测和成像

近红外（NIR）窗口的荧光成像由于其穿透深度和对比度高，在临床诊断和治疗方面具有巨大潜力。在这里，合成了一种基于 NIR 半花青的探针 ( CyP )，用于对活细胞和动物中的 Hg ^{2+进行选择性检测和成像。}以刚性呫吨结构为电子供体，三甲基二氢吲哚为电子受体，二苯基硫代膦酰氯为识别元素，探针CyP可特异性响应Hg ²⁺并转化为活化的供体-π-受体（D-π-A）在 710 nm 处具有最大 NIR 发射的图案。细胞和动物成像实验表明，探针CyP可以被 Hg ^2+激活。并且在成像中具有良好的浓度依赖性。同时，动物成像实验表明，激活的CyP探针表现出更高的组织穿透深度和时空分辨率。因此，CyP探针可能是监测和直观评估生命系统中Hg ^{2+的有用工具。}

更新日期：2022-06-07

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