Abstract

Candida krusei is a candidiasis etiological agent of relevance in the clinical setting because of its intrinsic resistance to fluconazole. Also, it has opened up new paths in the area of alternative therapeutic techniques. This project demonstrated the effects of diphenyl diselenide (PhSe)₂ and p-cloro diphenyl diselenide (pCl-PhSe)₂, two organochalcogen compounds, on relevant virulence factors for the early stage of the C. krusei host interaction and infection process. Both compounds inhibited adherence of C. krusei to both polystyrene surfaces and cervical epithelial cells and biofilm formation; the structure of the biofilm was also changed in a dose-dependent manner. In addition, both compounds inhibited C. krusei growth, but (PhSe)₂ significantly increased the time duration of the lag phase and delayed the start of the exponential phase in growth kinetics. (PhSe)₂ has more potential antifungal activity than (pCl-PhSe)₂ in inhibiting the adherence to epithelial cells, biofilm formation, and growth of C. krusei.

摘要

克氏念珠菌是一种与临床环境相关的念珠菌病病原体，因为它对氟康唑具有内在的耐药性。此外，它还开辟了替代治疗技术领域的新途径。本项目展示了二苯基二硒化物 (PhSe) ₂和对氯二苯基二硒化物 ( p Cl-PhSe) _{2这两种有机硫属化合物对}克氏梭菌宿主相互作用和感染过程早期相关毒力因子的影响。两种化合物都抑制C. krusei的粘附对聚苯乙烯表面和宫颈上皮细胞和生物膜形成；生物膜的结构也以剂量依赖性方式发生变化。此外，这两种化合物都抑制了克氏梭菌的生长，但 (PhSe) ₂显着增加了滞后期的持续时间并延迟了生长动力学中指数期的开始。(PhSe) ₂在抑制 C. krusei 的上皮细胞粘附、生物膜形成和生长方面具有比 ( p Cl-PhSe) _{2更高的潜在抗真菌活性}。