Calibration is the primary step in most of the instrumental analytical techniques. Separate calibration standard solutions of Mn, Co were prepared by dissolution of MnSO₄·H₂O and Co metal in HNO₃ media. Neutron activation analysis and energy-dispersive X-ray fluorescence spectrometry were used for assay, homogeneity and stability assessments of these solutions. During the whole preparation, assessment processes, all care was taken to maintain an unbroken chain of comparisons, to establish the traceability of the present solutions to NIST reference standard solutions. The role of appropriate concentration range for multi-point calibration procedures was explicit by closely monitoring the intercept and slope of the least square curve. Accuracies of the measurements for different concentration ranges were compared in terms of the % error in the final calculated concentrations for quality control standards. Atomic absorption spectrometry and inductively coupled plasma optical emission spectrometry were used to assess the impurity profiles in the in-house calibration standard solutions. The values of Co and Mn were assigned according to ISO Guide 80:2014 guidelines.

校准是大多数仪器分析技术的主要步骤。_{将 MnSO 4} ·H ₂ O 和 Co 金属溶解在 HNO _{3中制备 Mn、Co 的单独校准标准溶液}媒体。中子活化分析和能量色散 X 射线荧光光谱法用于这些溶液的测定、均匀性和稳定性评估。在整个准备、评估过程中，我们都小心翼翼地保持一个完整的比较链，以建立当前解决方案与 NIST 参考标准溶液的可追溯性。通过密切监测最小二乘曲线的截距和斜率，多点校准程序的适当浓度范围的作用是明确的。根据质量控制标准的最终计算浓度的百分比误差比较不同浓度范围的测量精度。原子吸收光谱法和电感耦合等离子体发射光谱法用于评估内部校准标准溶液中的杂质分布。Co 和 Mn 的值是根据 ISO 指南 80:2014 指南分配的。