Filamentous fungi occupy a uniquely favorable position in the bioproduction of organic acids. Intracellular stress is the main stimulator in filamentous fungi to produce and accumulate organic acids with high flux. However, stress can affect the physiological activities of filamentous fungi, thereby deteriorating their fermentation performance. Herein, we report that peptide supplementation during Rhizopus oryzae fermentation significantly improved fumaric acid production. Specifically, fumaric acid productivity was elevated by approximately 100%, fermentation duration was shortened from 72 to 36 h, while maintaining the final titer. Furthermore, transcriptome profile analysis and biochemical assays indicated that the overall capabilities of the stress defense systems (enzymatic and nonenzymatic) were significantly improved in R. oryzae. Consequently, glycolytic metabolism was distinctly enhanced, which eventually resulted in improved fumaric acid production and reduced fermentation duration. We expect our findings and efforts to provide essential insights into the optimization of the fermentation performance of filamentous fungi in industrial biotechnology and fermentation engineering.

中文翻译：

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在有机酸生物生产过程中，肽补充剂可缓解压力并增强丝状真菌的糖酵解通量

丝状真菌在有机酸的生物生产中占有独特的有利地位。细胞内应激是丝状真菌产生和积累高通量有机酸的主要刺激因素。然而，压力会影响丝状真菌的生理活动，从而降低其发酵性能。在此，我们报告了米根霉期间的肽补充发酵显着提高了富马酸的产量。具体来说，富马酸生产率提高了大约 100%，发酵持续时间从 72 小时缩短到 36 小时，同时保持最终滴度。此外，转录组谱分析和生化分析表明，稻瘟病菌的应激防御系统（酶促和非酶促）的整体能力显着提高。因此，糖酵解代谢明显增强，最终导致富马酸产量提高并缩短发酵持续时间。我们希望我们的发现和努力为工业生物技术和发酵工程中丝状真菌发酵性能的优化提供重要的见解。