Scanning electron microscopy for identification of local strain of Aspergillus Parasiticus and its larvicidal efficacy against Aedes Aegypti and non-target toxicity testing on fingerlings of Hypophthalmichthys Molitrix,Microscopy Research and Technique

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Scanning electron microscopy for identification of local strain of Aspergillus Parasiticus and its larvicidal efficacy against Aedes Aegypti and non-target toxicity testing on fingerlings of Hypophthalmichthys Molitrix
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-06-06 , DOI: 10.1002/jemt.24176
Amina Abrar ₁ , Moneeza Abbas ₁ , Salma Mehmood ₁ , Nadia Ghani ₁ , Ammara Fatima ₁ , Rabia Shahzadi ₁

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Scanning electron microscopy proved to be helpful in identification of Aspergillus parasiticus. A. parasiticus has a worldwide occurrence with high entomopathogenic efficacy against Aedes aegypti. However, assessment of pathogenic effects of entomopathogenic fungi on non-target organisms is required to evaluate its use as bio control agent. Aim of the present research is to study the parricidal activity of the local strain of A. parasiticus (MK371710) against A. aegypti and its toxicity assessment against aquatic model organism Hypophthalmichthys molitrix. A. parasiticus was isolated from the soil of Jallo Park, Lahore. The larvicidal effect of A. parasiticus was evaluated against fourth instar larvae of A. aegypti. Hundred percent of mortality of larvae was observed after exposure to 1 × 10⁷ conidia/ml of fungal suspension after 72 h post treatment. The LC₅₀ value of A. parsiticus in 24 h post treatment and 48 h post treatment was recorded as 1.0 × 10⁷conidia/ml 2.99 × 10⁵conidia/ml, respectively. However, an in-depth understanding safety to non-target organisms is necessary, if we are to properly control the action of these entomopathogenic fungi under natural conditions. For the toxicity assessment fingerlings of H. molitrix were exposed to conidial suspensions of A. parasiticus. Eye pop (64%), fin hemorrhage (33%), and scale infection (30%) were the major morphological effects observed during the study. Results reveal that although A. parasiticus is highly pathogenic to dengue vector but also have significant effects on organisms other than insects and its application as biological control agent requires safety considerations.

中文翻译：

扫描电镜鉴定寄生曲霉局部菌株及其对埃及伊蚊的杀幼虫效果及对下眼鱼鱼种的非靶标毒性试验

扫描电子显微镜证明有助于鉴定寄生曲霉。A. parasiticus在世界范围内流行，对埃及伊蚊具有很高的昆虫病原菌效力。然而，需要评估昆虫病原真菌对非目标生物的致病作用，以评估其作为生物防治剂的用途。本研究的目的是研究A. parasiticus (MK371710) 局部菌株对A. aegypti的杀寄生虫活性及其对水生模式生物Hypophthalmichthys molitrix 的毒性评估。A. parasiticus是从拉合尔 Jallo Park 的土壤中分离出来的。A. parasiticus的杀幼虫作用针对埃及伊蚊的第四龄幼虫进行了评估。在处理后 72 小时后暴露于 1 × 10 ⁷分生孢子/ml 的真菌悬浮液后，观察到 100% 的幼虫死亡率。A. parsiticus处理后24小时和处理后48小时的LC ₅₀值分别记录为1.0×10 ⁷分生孢子/ml和2.99×10 ⁵分生孢子/ml。然而，如果我们要在自然条件下正确控制这些昆虫病原真菌的作用，就必须深入了解对非目标生物的安全性。为了进行毒性评估，将H. molitrix鱼种暴露于A. parasiticus的分生孢子悬浮液中. 眼球突出（64%）、鳍出血（33%）和鳞片感染（30%）是研究期间观察到的主要形态学影响。结果表明，虽然A. parasiticus对登革热载体具有高致病性，但对昆虫以外的生物也有显着影响，其作为生物防治剂的应用需要考虑安全性。

更新日期：2022-06-06

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