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Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2022-06-06 , DOI: 10.1002/anie.202201458 Oliver J Hale 1 , Helen J Cooper 1
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2022-06-06 , DOI: 10.1002/anie.202201458 Oliver J Hale 1 , Helen J Cooper 1
Affiliation
The integral membrane protein assembly Aquaporin-0 was analysed by native ambient mass spectrometry of eye lens tissue. Membrane protein assemblies were solubilised by detergent micelles. Spatially-resolved analysis for mapping protein assembly distributions, accurate intact mass determination, and the identification of assemblies approx. 100 kDa in molecular weight are demonstrated.
中文翻译:
对直接来自晶状体组织的完整膜蛋白组装体和可溶性蛋白组装体进行天然环境质谱分析
通过眼晶状体组织的天然环境质谱分析完整膜蛋白组装体 Aquaporin-0。膜蛋白组装体通过去污剂胶束溶解。用于绘制蛋白质组装分布图、精确完整质量测定以及约组装体鉴定的空间分辨分析。证明分子量为 100 kDa。
更新日期:2022-06-06
中文翻译:
对直接来自晶状体组织的完整膜蛋白组装体和可溶性蛋白组装体进行天然环境质谱分析
通过眼晶状体组织的天然环境质谱分析完整膜蛋白组装体 Aquaporin-0。膜蛋白组装体通过去污剂胶束溶解。用于绘制蛋白质组装分布图、精确完整质量测定以及约组装体鉴定的空间分辨分析。证明分子量为 100 kDa。