Rapid, sensitive and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great need for effective quarantining and treatment. Real-time reverse-transcription polymerase chain reaction requiring thermocyling has been commonly used for diagnosis of SARS-CoV-2 though it may take two to 4 h before lengthy sample pretreatment process and require bulky apparatus and well-trained personnel. Since multiple reverse transcription loop-mediated isothermal amplification (multiple RT-LAMP) process without thermocycling is sensitive, specific and fast, an electromagnetically-driven microfluidic chip (EMC) was developed herein to lyse SARS-CoV-2 viruses, extract their RNAs, and perform qualitative analysis of three marker genes by on-chip multiple RT-LAMP in an automatic format within 82 min at a limit of detection of only ∼5000 copies per reaction (i.e. 200 virus/ $\mathrm{\mu L})$. This compact EMC may be especially promising for SARS-CoV-2 diagnostics in resource-limited countries.

快速、灵敏和准确地诊断严重急性呼吸综合征冠状病毒2（SARS-CoV-2）对于有效的隔离和治疗非常必要。需要热循环的实时逆转录聚合酶链反应已普遍用于诊断 SARS-CoV-2，但在漫长的样品预处理过程之前可能需要 2 至 4 小时，并且需要笨重的设备和训练有素的人员。由于无需热循环的多重逆转录环介导的等温扩增（多重 RT-LAMP）过程灵敏、特异且快速，本文开发了一种电磁驱动的微流控芯片（EMC）来裂解 SARS-CoV-2 病毒，提取其 RNA，并通过片上多重 RT-LAMP 以自动格式在 82 分钟内对三个标记基因进行定性分析，每个反应的检测限仅为 ∼5000 个拷贝（即 200 个病毒/$\mathrm{<span\; data-immersive-translate-walked="b788677f-d250-4f83-916d-868cbed93893">微升</span>}<span\; data-immersive-translate-walked="b788677f-d250-4f83-916d-868cbed93893">）</span>$ 。这种紧凑型 EMC 对于资源有限国家的 SARS-CoV-2 诊断尤其有前景。