Plant and Soil ( IF 3.9 ) Pub Date : 2022-06-04 , DOI: 10.1007/s11104-022-05519-w Eva Oburger 1 , Christiana Staudinger 1, 2 , Andreea Spiridon 1 , Vera Benyr 1 , David Aleksza 1 , Walter Wenzel 1 , Michael Santangeli 1, 2
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Purpose
Root exudates are key components driving belowground interaction between plant, microbes and soil. High-end analytical approaches provide advanced insights into exudate metabolite diversity, however, the amount of total carbon (C) released by roots should always be determined as the most basic parameter when characterizing root exudation as it (i) provides quantitative information of C exuded into the surrounding soil and (ii) allows to relate the abundance of individual exudate compounds to total C released. Here we propose a simple and quick, spectrophotometry-based method to quantify total dissolved organic carbon (DOC) concentration in exudation samples that is based on measuring the absorption of a pre-filtered but otherwise untreated exudate sample at 260 nm (DOC260).
Method
Exudate samples collected from different grass genotypes (Zea mays, Oryza sativa, Hordeum vulgare) grown in various experimental settings (soil, hydroponic) were analysed with the DOC260 assay and results were compared with C concentrations obtained by liquid TOC-analyser.
Conclusion
We demonstrated that the DOC260 method allowed for quick and inexpensive measurements of total dissolved organic carbon concentrations in exudate samples from grass species grown under nutrient sufficient as well as under P deficient conditions. Interestingly, DOC260 failed to predict DOC concentrations in exudate samples from plants grown under Zn and Fe deficiency suggesting a strong shift in metabolite composition under micronutrient deficiency. Even though the applicability of the DOC260 method remains to be tested on exudate samples originating from dicots and plants exposed to other environmental stresses (e.g. pathogen attack, heavy metal stress, etc), it will help to increase our understanding of root exudation and related rhizosphere processes in the future.
中文翻译：
一种快速简便的分光光度法测定草种根系分泌物样品中总碳浓度
目的
根系分泌物是推动植物、微生物和土壤之间地下相互作用的关键成分。高端分析方法提供了对渗出物代谢物多样性的高级见解，但是，在表征根部渗出物时，应始终将根释放的总碳 (C) 量确定为最基本的参数，因为它 (i) 提供了渗出的 C 的定量信息进入周围的土壤和（ii）允许将单个渗出物化合物的丰度与释放的总碳联系起来。在这里，我们提出了一种简单快速的基于分光光度法的方法来量化渗出液样品中的总溶解有机碳 (DOC) 浓度，该方法基于测量预过滤但未经处理的渗出液样品在 260 nm (DOC 260 ) 处的吸收。
方法
使用 DOC 260测定法分析从在各种实验环境（土壤、水培）中生长的不同草基因型（玉米、水稻、大麦）收集的渗出液样品，并将结果与液体 TOC 分析仪获得的 C 浓度进行比较。
结论
我们证明了 DOC 260方法可以快速、廉价地测量在营养充足和 P 缺乏条件下生长的草种的渗出液样品中的总溶解有机碳浓度。有趣的是，DOC 260未能预测在缺锌和缺铁条件下生长的植物的渗出液样品中的 DOC 浓度，这表明在微量营养素缺乏条件下代谢物组成发生了强烈变化。尽管 DOC 260的适用性方法仍有待对来自暴露于其他环境胁迫（例如病原体攻击、重金属胁迫等）的双子叶植物和植物的渗出物样本进行测试，这将有助于我们在未来增加对根系分泌物和相关根际过程的理解。
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