Virulence of the entomopathogenic nematode, Heterorhabditis indica against larvae (3rd, 4th and 5th larval instars) and pupae of citrus fruit piercing moth, Eudocima materna L. (Lepidoptera: Erebidae) was evaluated under laboratory conditions. The experiments included pathogenicity assessment of H. indica against larvae and pupae using a range of concentrations (10, 25, 50, 100 and 200 infective juveniles (IJs) larva−1 and pupa−1) as well as evaluation of their reproductive potential on different larval instars with different concentrations (50, 100, 200, 400 and 600 IJs larva−1). Application of increasing concentrations of IJs induced higher mortality on larval instars and pupae with mean mortality ranging from 26.6 to 100% (LC50- 3rd (14.43 IJs larva−1), 4th (17.08 IJs larva−1), 5th (23.63 IJs larva−1)) and 10–70% (LC50- 85.91 IJs pupa−1) after 48 h post-exposure, respectively. H. indica successfully reproduced in the 3rd, 4th and 5th larval instars of E. materna and their offspring emerged from the cadavers. The highest reproduction was recorded in 5th instar larvae (1,082,855 IJs larva−1) at 600 IJs larva−1 in E. materna. The present study suggests that H. indica strain had the potential for biological control of E. materna.

中文翻译：

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Heterorhabditis indica 对 Eudocima materna L. (Lepidoptera, Erebidae) 发育阶段的致病性

在实验室条件下评估了昆虫病原线虫 Heterorhabditis indica 对柑橘果刺蛾 Eudocima materna L. (Lepidoptera: Erebidae) 幼虫（第 3、4 和 5 龄幼虫）和蛹的毒力。实验包括使用一系列浓度（10、25、50、100 和 200 只感染性幼虫 (IJs) larva-1 和 pupa-1）对 H. indica 对幼虫和蛹的致病性评估，以及评估它们在不同浓度的不同幼虫龄期（50、100、200、400 和 600 IJs larva-1）。增加 IJs 浓度的应用导致幼虫龄和蛹的死亡率更高，平均死亡率为 26.6% 至 100%（LC50-3rd（14.43 IJs larva-1）、4th（17.08 IJs larva-1）、5th（23.63 IJs larva-1） 1)) 和 10–70% (LC50-85. 91 IJs pupa-1) 分别在暴露后 48 小时后。H. indica 在 E. materna 的第 3、第 4 和第 5 龄幼虫中成功繁殖，它们的后代从尸体中出现。在 E. materna 中，在 600 IJs larva-1 的 5 龄幼虫（1,082,855 IJs larva-1）中记录的繁殖率最高。本研究表明 H. indica 菌株具有生物防治 E. materna 的潜力。