Antagonistic Bacillus sp. (Bacillus amyloliquefaciens QSB-6, Bacillus vallismortis HSB-2, Bacillus licheniformis XNRB-3, Bacillus velezensis XC1, Bacillus siamensis 2RS-9, Bacillus subtilis 4R-5, and Bacillus atrophaeus 9R-5) with common pathogens (Fusarium oxysporum, Fusarium solani, Fusarium proliferatum, Fusarium moniliforme, Alternaria alternata, Rhizoctonia solani, Albifimbria verrucaria, Phoma asparagi, and Phytophthora cactorum) which cause apple replant disease (ARD) were screened, and a composite microbial culture was prepared. The composite bacterial broth fermentation conditions were optimized by single factor and orthogonal experiments. When 2.6 × 10⁹ amount composite microbial culture (at CFU·g⁻¹) was added to the soil together with manure carrier (cow manure:straw=3:1) (T3), it significantly increased the biomass of Malus hupehensis Rehd. seedlings and young apple trees (Fuji New2001/M9T337), the population of soil bacteria, and activities of antioxidant enzymes as well as soil enzymes, and significantly reduced the population of soil fungi and the abundance of Fusarium sp. In conclusion, composite microbial culture could thus be used as a biological control agent for the management of ARD.

拮抗芽孢杆菌 ( Bacillus amyloliquefaciens QSB-6, Bacillus vallismortis HSB-2, Bacillus licheniformis XNRB -3, Bacillus velezensis XC1, Bacillus siamensis 2RS-9, Bacillus subtilis 4R-5, and Bacillus atrophaeus 9R-5) 与常见病原体（尖孢镰刀菌、镰刀菌）茄属植物、Fusarium proliferatum、串珠镰刀菌、链格孢、立枯丝核菌、Albifimbria verrucaria、Phoma asparagi和Phytophthora cactorum) 筛选出导致苹果再植病 (ARD) 的微生物，并制备了复合微生物培养物。通过单因素试验和正交试验对复合菌液发酵条件进行优化。当2.6×10 ⁹量的复合微生物培养物（在CFU·g ^-1）与粪肥载体（牛粪：秸秆=3：1）（T3）一起加入土壤时，显着增加了湖北海棠的生物量。苗和苹果幼树（Fuji New2001/M9T337）、土壤细菌数量、抗氧化酶活性和土壤酶活性，显着降低土壤真菌数量和镰刀菌丰度sp。总之，复合微生物培养因此可以用作控制急性呼吸道疾病的生物控制剂。