Rotenone (ROT) is a widely used natural pesticide, and its effect on growth and developmental toxicity remain unclear. In the present study, the effects of ROT exposure on the reproductive structure and function of the female Drosophila melanogaster and third instar larvae were investigated. ROT exposure on female Drosophila melanogaster resulted in developmental inhibition and ovarian abnormality, which were evident from the disruptive growth of border cells as well as morphological changes in the orientation of nurse cells during the 9th-10th stage of developing egg chamber of in the Drosophila ovary. Other abnormalities, such as, altered developmental gene expression (Osk, Grk, Nos, Bic-d), inhibition in the kinesin motor protein level (KIF-5B), increased caspases activities (Caspase 3, 8, & 9) and apoptosis were also observed. Subsequently, ROT treated larvae exhibited behavioral deficits and delay in developmental time. The above findings demonstrate that the exposure of ROT causes developmental toxicity in Drosophila melanogaster.

鱼藤酮（ROT）是一种广泛使用的天然农药，其对生长和发育毒性的影响尚不清楚。在本研究中，研究了 ROT 暴露对雌性黑腹果蝇和三龄幼虫生殖结构和功能的影响。雌性黑腹果蝇的 ROT 暴露导致发育抑制和卵巢异常，这从果蝇卵巢发育卵室的第 9-10 阶段边缘细胞的破坏性生长以及护士细胞方向的形态学变化中可以看出。. 其他异常，例如发育基因表达改变（Osk、Grk、Nos、Bic-d)，还观察到驱动蛋白运动蛋白水平 (KIF-5B) 的抑制、半胱天冬酶活性增加 (半胱天冬酶 3、8 和 9) 和细胞凋亡。随后，ROT 处理的幼虫表现出行为缺陷和发育时间延迟。上述发现表明，ROT 的暴露会导致黑腹果蝇的发育毒性。