Effective skin wound healing is a complex process involving anti-inflammation, fibrosis, matrix reconstruction, and angiogenesis. This work aimed to integrate the macrophage-mediated anti-inflammation and fibroblast-assisted matrix reconstruction for enhanced skin wound healing. Herein, we utilized the cytomembranes derived from repolarized M2 macrophages and fibroblasts to prepare the natural biologics. Results showed that the inflammatory M1 macrophages were repolarized to M2 phenotype by the M2 macrophage cytomembranes. As a consequence, the cytomembranes of M2 macrophage could facilitate the wound closure in mice. Furthermore, the addition of fibroblast membranes to the macrophage cytomembranes contributed to a better matrix reconstruction, neovascularization and angiogenesis. Next, we used a transforming growth factor-β (TGF-β) inhibitor to attenuate cutaneous scar formation. Therefore, our modality could promote skin wound healing and effectively suppress scar formation in the preclinical murine skin wounds. The cytomembrane biologics might provide a biocompatible and versatile tool for wound healing.

中文翻译：

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生物制造的巨噬细胞和成纤维细胞膜协同促进皮肤伤口愈合


有效的皮肤伤口愈合是一个复杂的过程，涉及抗炎、纤维化、基质重建和血管生成。这项工作旨在整合巨噬细胞介导的抗炎和成纤维细胞辅助的基质重建，以增强皮肤伤口愈合。在此，我们利用复极化的 M2 巨噬细胞和成纤维细胞来源的细胞膜来制备天然生物制剂。结果表明，炎症性 M1 巨噬细胞被 M2 巨噬细胞细胞膜复极化为 M2 表型。因此，M2 巨噬细胞的细胞膜可以促进小鼠伤口的闭合。此外，在巨噬细胞膜上添加成纤维细胞膜有助于更好的基质重建、新血管形成和血管生成。接下来，我们使用转化生长因子-β (TGF-β) 抑制剂来减轻皮肤疤痕形成。因此，我们的方法可以促进皮肤伤口愈合并有效抑制临床前小鼠皮肤伤口的疤痕形成。细胞膜生物制剂可能为伤口愈合提供生物相容性和多功能工具。