In this study, a target-responsive release Surface-enhanced Raman scattering (SERS) sensor was developed for sensitive detection of tetracycline (TTC) using aptamer-gated HP-UiO-66-NH₂ nanochannel strategy. The hierarchically porous zirconium-based metal-organic frameworks (HP-UiO-66-NH₂ MOFs) with high surface areas and the conjugated π-electron system were first synthesized and served as the nanocontainers. The methylene blue (MB) and gold nanoparticles (AuNPs) were then loaded in the pores of HP-UiO-66-NH₂ as the signal probes and capping agent, respectively. Thereafter, aptamer was assembled on the surface of HP-UiO-66-NH₂ based on the π-π stacking interaction. In the presence of TTC, the aptamer “molecular gate” was opened, resulting in the “cargo release” of MB and AuNPs. Hence, the amount of TTC could be determined by monitoring the change of SERS intensity of the supernatant. Under the optimal conditions, a good linear correlation between SERS intensity (886 cm⁻¹) and TTC concentration was observed with the concentration from 0.01 to 10000 ng/mL, resulting in a relatively low detection limit of 0.01 ng/mL. Moreover, this method showed a promising practical application in spiked real samples (milk and pork) with recoveries of 93.23–108.79%, which confirmed its great potential in antibiotic detection.

在这项研究中，开发了一种目标响应释放表面增强拉曼散射 (SERS) 传感器，用于使用适体门控 HP-UiO-66-NH ₂纳米通道策略灵敏检测四环素 (TTC)。首先合成了具有高表面积的分级多孔锆基金属有机骨架（HP-UiO-66-NH ₂ MOFs）和共轭π电子体系并用作纳米容器。然后将亚甲蓝 (MB) 和金纳米粒子 (AuNPs) 分别加载到 HP-UiO-66-NH ₂的孔中作为信号探针和封端剂。此后，适体组装在HP-UiO-66-NH _2表面基于 π-π 堆叠相互作用。在 TTC 存在下，适体“分子门”打开，导致 MB 和 AuNPs 的“货物释放”。因此，可以通过监测上清液 SERS 强度的变化来确定 TTC 的量。在最佳条件下，SERS 强度 (886 cm ^-1 ) 与 TTC 浓度之间具有良好的线性相关性，浓度为 0.01 至 10000 ng/mL，导致检测限相对较低，为 0.01 ng/mL。此外，该方法在加标的真实样品（牛奶和猪肉）中显示出有前景的实际应用，回收率为 93.23-108.79%，这证实了其在抗生素检测中的巨大潜力。