We have designed and synthesised a novel fluorescent probe with a tetraphenylethylene (TPE) scaffold as an active fluorescent unit and thiosemicarbazide (TSC) group as a recognition unit. The probe, TPE–TSC, exhibited superior selectivity towards hypochlorite (ClO⁻) with a low limit of detection (2.0 nM). It also demonstrated a turn-off response for a brief period (<30 s) via an oxidation reaction. Furthermore, high-resolution mass spectrometry (HRMS) revealed that TPE–TSC reacted with ClO⁻ by forming a carboxylic acid moiety in nearly 100% aqueous environments. More significantly, the probe detected ClO⁻ in disinfectant, spiked milk samples, and spiked water samples. In all, TPE–TSC proposes an optimistic approach precisely for the determining the quality of milk and water contaminated with ClO⁻ and trace amounts of ClO⁻ in disinfectants.

我们设计并合成了一种新型荧光探针，其以四苯乙撑（TPE）支架为活性荧光单元，氨基硫脲（TSC）基团为识别单元。探针TPE-TSC对次氯酸盐 (ClO ^- ) 表现出优异的选择性，检测限低 (2.0 nM)。它还通过氧化反应展示了短暂（<30 秒）的关闭响应。此外，高分辨率质谱 (HRMS) 显示TPE-TSC通过在几乎 100% 的水性环境中形成羧酸部分与 ClO ^-反应。更重要的是，该探针检测到消毒剂、加标牛奶样品和加标水样中的二氧化氯^。在所有，TPE-TSC提出了一种乐观的方法，可以准确地确定被 ClO ^-和消毒剂中微量ClO ^-污染的牛奶和水的质量。