Electrochemical Impedance-Based Biosensors for the Label-Free Detection of the Nucleocapsid Protein from SARS-CoV-2,ACS Sensors

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Electrochemical Impedance-Based Biosensors for the Label-Free Detection of the Nucleocapsid Protein from SARS-CoV-2
ACS Sensors ( IF 8.2 ) Pub Date : 2022-06-02 , DOI: 10.1021/acssensors.2c00317
Hana Cho _{1,

2} , Suhyun Shim ₁ , Won Woo Cho ₃ , Sungbo Cho ₄ , Hanseung Baek ₅ , Sang-Myung Lee ₃ , Dong-Sik Shin _{1,

2}

Affiliation

Diagnosis of coronavirus disease (COVID-19) is important because of the emergence and global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time polymerase chain reaction (PCR) is widely used to diagnose COVID-19, but it is time-consuming and requires sending samples to test centers. Thus, the need to detect antigens for rapid on-site diagnosis rather than PCR is increasing. We quantified the nucleocapsid (N) protein in SARS-CoV-2 using an electro-immunosorbent assay (El-ISA) and a multichannel impedance analyzer with a 96-interdigitated microelectrode sensor (ToAD). The El-ISA measures impedance signals from residual detection antibodies after sandwich assays and thus offers highly specific, label-free detection of the N protein with low cross-reactivity. The ToAD sensor enables the real-time electrochemical detection of multiple samples in conventional 96-well plates. The limit of detection for the N protein was 0.1 ng/mL with a detection range up to 10 ng/mL. This system did not detect signals for the S protein. While this study focused on detecting the N protein in SARS-CoV-2, our system can also be widely applicable to detecting various biomolecules involved in antigen–antibody interactions.

中文翻译：

用于无标记检测 SARS-CoV-2 核衣壳蛋白的电化学阻抗生物传感器

由于严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的出现和全球传播，冠状病毒病 (COVID-19) 的诊断非常重要。实时聚合酶链反应 (PCR) 广泛用于诊断 COVID-19，但它非常耗时，并且需要将样本发送到测试中心。因此，检测抗原以进行快速现场诊断而不是 PCR 的需求正在增加。我们使用电免疫吸附测定 (El-ISA) 和带有 96 叉指微电极传感器 (ToAD) 的多通道阻抗分析仪对 SARS-CoV-2 中的核衣壳 (N) 蛋白进行了定量。El-ISA 可测量夹心测定后残留检测抗体的阻抗信号，从而以低交叉反应性提供高度特异性、无标记的 N 蛋白检测。ToAD 传感器能够对传统 96 孔板中的多个样品进行实时电化学检测。N 蛋白的检测限为 0.1 ng/mL，检测范围高达 10 ng/mL。该系统没有检测到 S 蛋白的信号。虽然本研究重点检测 SARS-CoV-2 中的 N 蛋白，但我们的系统也可广泛适用于检测参与抗原抗体相互作用的各种生物分子。

更新日期：2022-06-02

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