Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry,Analyst

当前位置： X-MOL 学术 › Analyst › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry
Analyst ( IF 3.6 ) Pub Date : 2022-06-02 , DOI: 10.1039/d2an00224h
Tianqi Gao ₁ , Aneirin A Lott _{2,

3} , Fanran Huang ₁ , Rajendra Rohokale ₁ , Qingjiang Li ₁ , Hernando J Olivos ₄ , Sixue Chen _{2,

3} , Zhongwu Guo ₁

Affiliation

LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). It was found that different forms of GalNAc-LcGg4 carrying homologous (d16:1/18:0) and (d18:1/18:0) lipids were easily separated and identified using liquid chromatography (LC)-MS. In addition, like gangliosides, homologous lipid forms of GalNAc-LcGg4 showed the same fragmentation pattern, except for a uniform shift of their glycolipid product ions by a certain m/z number determined by the varied lipid structure. It was also disclosed that LcGg4 and its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, which are different only in the C4-configuration of their non-reducing end sugar residues, gave the same MS/MS product ions in similar relative intensities, as well as the same LC retention time, suggesting the challenge to differentiate epimeric GSLs by LC-MS. However, ion mobility spectrometry (IMS)-MS was able to efficiently separate and distinguish these epimers. This study has demonstrated the promise of IMS-MS for isomeric GSL characterization and the IMS-MS and LC-MS/MS combination for natural GSL analysis.

中文翻译：

离子迁移谱-质谱法对中性鞘糖脂 LcGg4 不同差向异构体的结构表征和分析

通过质谱 (MS) 研究了中性鞘糖脂 (GSL) 和癌症抗原 LcGg4、其差向异构体 GalNAc-LcGg4 和 GlcNAc-LcGg4 以及 GalNAc-LcGg4 的三种脂质形式。研究发现，携带同源 (d16:1/18:0) 和 (d18:1/18:0) 脂质的不同形式的 GalNAc-LcGg4 很容易使用液相色谱 (LC)-MS 进行分离和鉴定。此外，与神经节苷脂一样，GalNAc-LcGg4 的同源脂质形式显示出相同的碎裂模式，除了其糖脂产物离子按一定的m / z均匀移动之外。数量由不同的脂质结构决定。还公开了 LcGg4 及其差向异构体 GalNAc-LcGg4 和 GlcNAc-LcGg4（仅其非还原端糖残基的 C4 构型不同）也以相似的相对强度给出相同的 MS/MS 产物离子。相同的 LC 保留时间，表明通过 LC-MS 区分差向异构体 GSL 存在挑战。然而，离子迁移谱 (IMS)-MS 能够有效分离和区分这些差向异构体。这项研究证明了 IMS-MS 用于异构 GSL 表征以及 IMS-MS 和 LC-MS/MS 组合用于天然 GSL 分析的前景。

更新日期：2022-06-02

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11