Aims

There is a growing interest in the use of microbials in sustainable protected cultivation systems. A novel study on the influence of chili residue and plant growth-promoting rhizobacteria (PGPR) interaction was conducted to investigate the agronomic response of a greenhouse-grown leaf mustard (Brassica juncea L.), as well as the soil productivity.

Methods

Bacillus subtilis (B1), B. laterosporus (B2), or B. amyloliquefaciens (B3) was inoculated on soil containing chili residue in a solar greenhouse. Other treatments were chili residue alone (NoPGPR) and chemical fertilizer (ChemFert). The crop photosynthesis, growth, yield, and quality, the soil chemical properties, microbial biomass, and the rhizospheric bacterial community were evaluated.

Results

Residue and PGPR synergy significantly enhanced the leaf mustard’s productivity and soil quality than NoPGPR and ChemFert. B1, B2, and B3 improved the crop yield by 51–63% than NoPGPR and ChemFert. They also increased the net photosynthetic rate, plant uptakes of N, P, K, Mg, Ca, Mn, Zn, and soil microbial quotient than NoPGPR and ChemFert, which comparatively elicited lower leaf chlorophyll-a, total soluble solids, nutrients uptakes compared to the PGPR treatments. Residue and B. laterosporus synergy markedly improved the soil organic carbon, total nitrogen, microbial carbon contents, and the microbial quotient. Further, 16S rRNA high throughput sequencing revealed that inoculating chili residue with B. laterosporus more consistently enriched the rhizospheric bacterial community composition at the phylum and genus levels, although with a lower diversity.

Conclusions

Inoculating chili residue with B. laterosporus is a strategy with immense benefits as a bio-fertilizer for sustainable vegetable production, which also renders soil ecological services.

中文翻译：

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辣椒渣和侧孢芽孢杆菌的协同作用影响太阳能温室中的土壤细菌微生物组和芥菜（Brassica juncea L.）的农艺性能

目标

人们对在可持续受保护的栽培系统中使用微生物的兴趣日益浓厚。一项关于辣椒渣和植物促生根际细菌 (PGPR) 相互作用影响的新研究旨在调查温室种植的芥菜 ( Brassica juncea L.) 的农艺响应以及土壤生产力。

方法

在日光温室中，将枯草芽孢杆菌(B1)、侧孢芽孢杆菌 ( B2) 或解淀粉芽孢杆菌(B3) 接种到含有辣椒残渣的土壤上。其他处理是单独的辣椒渣（NoPGPR）和化肥（ChemFert）。对作物的光合作用、生长、产量和品质、土壤化学性质、微生物生物量和根际细菌群落进行了评价。

结果

与 NoPGPR 和 ChemFert 相比，残留物和 PGPR 的协同作用显着提高了芥菜的生产力和土壤质量。B1、B2 和 B3 比 NoPGPR 和 ChemFert 提高了 51-63% 的作物产量。与 NoPGPR 和 ChemFert 相比，它们还增加了净光合速率、植物对 N、P、K、Mg、Ca、Mn、Zn 和土壤微生物商的吸收，这导致相对较低的叶片叶绿素-a、总可溶性固体、养分吸收到 PGPR 治疗。残渣与侧孢芽孢杆菌的协同作用显着提高了土壤有机碳、全氮、微生物碳含量和微生物商。此外，16S rRNA 高通量测序表明，用B.尽管多样性较低，但更一致地丰富了门和属水平的根际细菌群落组成。

结论

用B. laterosporus接种辣椒残渣是一种具有巨大效益的策略，可作为可持续蔬菜生产的生物肥料，还可以提供土壤生态服务。