Drug Delivery and Translational Research ( IF 5.7 ) Pub Date : 2022-06-01 , DOI: 10.1007/s13346-022-01176-9 Marwah Suliman Maashi 1, 2 , Shatha G Felemban 3 , Hibah Ali Almasmoum 4 , Mostafa Jarahian 5
|
The current study developed a biopolymer-based wound dressing by electrospinning of Nicaraven-loaded collagen solution. Firstly, collagen was dissolved in acetic acid, and then Nicaraven was added to the polymeric solution at three different concentrations of 2 w/w%, 4 w/w%, and 6 w/w%. The resulting solution was then electrospun. Various experiments were performed to characterize the produced wound dressings. In vitro studies showed that Nicaraven-loaded scaffolds were not toxic against L929 fibroblast cells and protected them against oxidative stress. Wound healing potential of different formulations of Nicaraven-loaded collagen wound dressings was studied in a rat model of the excisional diabetic wound. The study showed that the collagen/4% Nicaraven and collagen/6% Nicaraven wound dressings exhibited a significantly higher percentage of wound closure, the thickness of the epithelium, and collagen deposition compared with collagen/2% Nicaraven, collagen-only, and sterile gauze groups. Gene expression study showed that the developed wound dressings reduced the tissue expression levels of glutathione peroxidase, NFKβ, and matrix metalloproteinase 9 (MMP9) genes. In addition, in the wounds treated with collagen/4% Nicaraven and collagen/6% Nicaraven scaffolds, wound healing was associated with a higher tissue expression level of b-FGF, VEGF, and collagen type I genes. Overall, wound healing activity of collagen/4% Nicaraven and collagen/6% Nicaraven wound dressings was not significantly different. This study implies that collagen wound dressings incorporated with 4% and 6% Nicaraven can be considered a potential candidate to treat diabetic wounds in the clinic.
Graphical abstract
中文翻译：
载尼加拉文的电纺伤口敷料通过促血管生成和免疫调节功能促进糖尿病伤口愈合：一项临床前研究
目前的研究通过静电纺丝 Nicaraven 加载的胶原蛋白溶液开发了一种基于生物聚合物的伤口敷料。首先，将胶原蛋白溶解在乙酸中，然后将 Nicaraven 以 2 w/w%、4 w/w% 和 6 w/w% 三种不同浓度添加到聚合物溶液中。然后将所得溶液电纺丝。进行了各种实验来表征所产生的伤口敷料。体外研究表明，装载 Nicaraven 的支架对 L929 成纤维细胞没有毒性，并保护它们免受氧化应激。在糖尿病切除伤口的大鼠模型中研究了不同配方的载有 Nicaraven 的胶原蛋白伤口敷料的伤口愈合潜力。研究表明，与胶原蛋白/2% Nicaraven、纯胶原蛋白和无菌相比，胶原蛋白/4% Nicaraven 和胶原蛋白/6% Nicaraven 伤口敷料的伤口闭合百分比、上皮厚度和胶原蛋白沉积明显更高纱布组。基因表达研究表明，开发的伤口敷料降低了谷胱甘肽过氧化物酶、NFKβ 和基质金属蛋白酶 9 (MMP9) 基因的组织表达水平。此外，在用胶原蛋白/4% Nicaraven 和胶原蛋白/6% Nicaraven 支架治疗的伤口中，伤口愈合与 b-FGF、VEGF 和 I 型胶原蛋白基因的更高组织表达水平相关。总体而言，胶原蛋白/4% Nicaraven 和胶原蛋白/6% Nicaraven 伤口敷料的伤口愈合活性没有显着差异。
京公网安备 11010802027423号